Publications by authors named "Julia Libby"
Article Synopsis
- A study examined the effects of ten VEGF genes on Alzheimer's disease (AD) using single-nucleus transcriptome data from the prefrontal cortex of 424 participants to identify cell type-specific influences on AD endophenotypes.
- The analysis employed negative binomial mixed models, revealing associations between higher VEGF receptor expressions in specific cell types (microglia, endothelial cells, and oligodendrocytes) with increased amyloid beta load and worse cognitive performance in AD.
- Findings indicate that VEGFB may have a protective effect in neurons against Aβ accumulation, while changes in FLT1 and FLT4 are linked to poorer cognitive outcomes, underscoring the importance of cell-specific VEGF signaling in AD pathology.
Article Synopsis
- The study investigates the genetic factors contributing to Alzheimer's disease by analyzing tau deposition through a genome-wide association study involving 3,046 participants.
- It identifies the CYP1B1-RMDN2 locus as significantly linked to tau levels, with the variant rs2113389 explaining 4.3% of tau variation, while also correlating with cognitive decline.
- Findings suggest a connection between CYP1B1 expression and tau deposition, offering potential new avenues for Alzheimer's treatment and understanding its genetic basis.
The cell-type specific role of the vascular endothelial growth factors (VEGFs) in the pathogenesis of Alzheimer's disease (AD) is not well characterized. In this study, we utilized a single-nucleus RNA sequencing dataset from Dorsolateral Prefrontal Cortex (DLFPC) of 424 donors from the Religious Orders Study and Memory and Aging Project (ROS/MAP) to investigate the effect of 10 VEGF genes ( , and ) on AD endophenotypes. Mean age of death was 89 years, among which 68% were females, and 52% has AD dementia.
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- - Proteomics research has evolved significantly due to advancements in high-throughput LC-MS/MS technology and automated workflows, enabling the analysis of large sample sizes but necessitating effective quality control (QC) measures for reliable results.
- - A study analyzed 335 patient plasma samples using TMT 16-plex methods over 10 months, with 271 pooled QC results generated from a representative plasma sample to monitor instrument performance and normalize data across batches.
- - Key metrics were assessed to develop a robust LC-MS/MS QC workflow, providing guidelines for ongoing QC checks and real-time troubleshooting to optimize instrument performance and enhance data reliability in future studies.
Determining the genetic architecture of Alzheimer's disease (AD) pathologies can enhance mechanistic understanding and inform precision medicine strategies. Here, we performed a genome-wide association study of cortical tau quantified by positron emission tomography in 3,136 participants from 12 independent studies. The locus was associated with tau deposition.
View Article and Find Full Text PDFBackground: Both Alzheimer's disease (AD) genetic risk factors and indices of cognitive reserve (CR) influence risk of cognitive decline, but it remains unclear whether they interact. This study examined whether a CR index score modifies the relationship between AD genetic risk factors and long-term cognitive trajectories in a large sample of individuals with normal cognition.
Methods: Analyses used data from the Preclinical AD Consortium, including harmonized data from 5 longitudinal cohort studies.
The vascular endothelial growth factor (VEGF) family of genes has been implicated in the clinical development of Alzheimer's Disease (AD). A previous study identified associations between gene expression of VEGF family members in the prefrontal cortex and cognitive performance and AD pathology. This study explored if those associations were also observed in the blood.
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