Publications by authors named "Julia J Gevaert"
Background: Despite widespread study of dendritic cell (DC)-based cancer immunotherapies, the in vivo postinjection fate of DC remains largely unknown. Due in part to a lack of quantifiable imaging modalities, this is troubling as the amount of DC migration to secondary lymphoid organs correlates with therapeutic efficacy. Magnetic particle imaging (MPI) has emerged as a suitable modality to quantify in vivo migration of superparamagnetic iron oxide (SPIO)-labeled DC.
View Article and Find Full Text PDFPurpose: Magnetic particle imaging (MPI) is being explored in biological contexts that require accurate and reproducible quantification of superparamagnetic iron oxide nanoparticles (SPIONs). While many groups have focused on improving imager and SPION design to improve resolution and sensitivity, a few have focused on improving quantification and reproducibility of MPI. The aim of this study was to compare MPI quantification results by two different systems and the accuracy of SPION quantification performed by multiple users at two institutions.
View Article and Find Full Text PDFPurpose: Magnetic particle imaging (MPI) is being explored in biological contexts that require accurate and reproducible quantification of superparamagnetic iron oxide nanoparticles (SPIONs). While many groups have focused on improving imager and SPION design to improve resolution and sensitivity, few have focused on improving quantification and reproducibility of MPI. The aim of this study was to compare MPI quantification results by two different systems and the accuracy of SPION quantification performed by multiple users at two institutions.
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- - Magnetic particle imaging (MPI) and bioluminescence imaging (BLI) are combined to track cancer cell activity and assess cell viability in mice, specifically using Akaluc-expressing 4T1Br5 cells labeled with superparamagnetic iron oxide nanoparticles (SPIO).
- - The study showed that BLI indicated tumor growth over time, while MPI revealed a decrease in signal due to SPIO dilution as the tumor grew, allowing insights into tumor dynamics and cell behavior.
- - This multimodal imaging technique provides a more comprehensive understanding of cancer cell fate, supporting better evaluation of treatment methods and metastatic mechanisms.
Article Synopsis
- Stem cell therapies have great potential for treating various diseases, and tracking their location and viability after being administered is crucial for assessing patient responses and side effects.
- This study focused on engineering mesenchymal stem cells (MSCs) with a PET reporter gene and magnetic nanoparticles to enable imaging through different methods, including MPI and BLI, over a 30-day period in mice.
- The findings revealed that while MPI was effective for early detection of MSCs, PET provided a clearer signal for tracking these cells over a longer duration, highlighting the benefits of using both imaging techniques together for comprehensive monitoring.
The use of imaging to detect and monitor the movement and accumulation of cells in living subjects can provide significant insights that can improve our understanding of metastasis and guide therapeutic development. For cell tracking using Magnetic Resonance Imaging (MRI), cells are labeled with iron oxides and the effects of the iron on water provides contrast. However, due to low specificity and difficulties in quantification with MRI, other modalities and approaches need to be developed.
View Article and Find Full Text PDFPurpose: The purpose of this study was to evaluate magnetic particle imaging (MPI) as a method for the in vivo tracking of dendritic cells (DC). DC are used in cancer immunotherapy and must migrate from the site of implantation to lymph nodes to be effective. The magnitude of the ensuing T cell response is proportional to the number of lymph node-migrated DC.
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- Many labs are working on using cellular magnetic resonance imaging (MRI) with superparamagnetic iron oxide nanoparticles (SPIONs) and fluorine-19 (F) for tracking immune and stem cells in therapies.
- SPION-based MRI has high sensitivity but low specificity and cannot reliably quantify cell numbers, while F-based tracking has good specificity but poor sensitivity.
- Magnetic particle imaging (MPI) is a promising new technique that can directly detect SPIONs, potentially improving both sensitivity and specificity in cell tracking and enabling accurate quantification of SPION-labeled cells.