Two methods for one-point anchoring of a linear polysaccharide on a gold surface.

Two strategies to achieve a one-point anchoring of a hydrolyzed pullulan (P9000) on a gold surface are compared. The first strategy consists of forming a self-assembled monolayer of a 6-amino-1-hexanethiol (AHT) and then achieving reductive amination on the surface between the aminated surface and the aldehyde of the polysaccharide reductive end sugar. The second consists of incorporating a thiol function at the extremity of the pullulan (via the same reductive amination), leading to P9000-AHT and then immobilizing it on gold by a spontaneous reaction between solid gold and thiol.