Publications by authors named "Julia E Rosenberger"
Article Synopsis
- The study introduces ligand-directed catalysts that utilize red light to enable bioorthogonal chemistry within live cells, highlighting the ability to control chemical reactions through light activation.
- Silarhodamine dyes are repurposed as photocatalysts, demonstrating high compatibility with cells and minimal byproducts, while other conjugates are used to target specific cell components like the nucleus and microtubules.
- The research shows that a novel photocage can release chemical compounds within cells, effectively promoting microtubule destabilization, which can be monitored in real-time using confocal microscopy.
Article Synopsis
- Researchers are exploring silicon-Rhodamine compounds (SiRs) as photocatalysts that use 660 nm light to enable bioorthogonal chemical reactions in biological systems, addressing limitations of traditional singlet oxygen sensitization.
- SiR derivatives, particularly Janelia Fluor-SiR dyes, effectively catalyze oxidation reactions without degrading target proteins, demonstrating high stability and potential for chemical modifications.
- This method allows for photoinducible hydrogel formation, applicable in 3D cell cultures and live mice, offering a cytocompatible approach with potential for broad applications in biological research.
trans-Cyclooctenes (TCOs) are essential partners in the fastest known bioorthogonal reactions, but current synthetic methods are limited by poor diastereoselectivity. Especially hard to access are hydrophilic TCOs with favorable physicochemical properties for live cell or in vivo experiments. Described is a new class of TCOs, "a-TCOs", prepared in high yield by stereocontrolled 1,2-additions of nucleophiles to trans-cyclooct-4-enone, which itself was prepared on a large scale in two steps from 1,5-cyclooctadiene.
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