Evaluation of bone regeneration in a critical size cortical bone defect in rat mandible using microCT and histological analysis.

Goal: Evaluate bone regeneration in a critical size bone defect model in the jaw of healthy rats as a function of gender and defect location.

Design: A series of microCT and histological studies were performed to evaluate the process of bone regeneration in rats with a mandibular critical size defect. Rats were placed in two groups according to gender and sorted in terms of bone defect location.

Viable pluripotent chick blastodermal cells can be maintained long term in an alkaline defined medium.

Most chicken embryonic cell culture methods call for neutral pH media of different natures, with disregard of the peculiar electrochemical environment in which avian embryos develop, with a 4 pH unit gradient across the thin blastoderm and the vitelline membrane. We report results of a culture system in alkaline media (pH >9) with atmospheric conditions. Blastodermal and blood cells, maintained for 8 wk with minor differentiation in the absence of the standard growth factors, developed a thick, mucoid-like matrix in which a large proportion of the cell mass grew embedded, with no direct contact to cultureware.

[Use of a composite prosthesis (PL-PU99) versus a biological prosthesis (Surgisis) in an experimental model of temporary abdominal closure].

Introduction: In some diseases of peritoneal origin, temporary closure of the abdominal cavity is required to avoid compartmental syndrome. This allows normal intra-abdominal pressure to be maintained and the fascial edges to be preserved for subsequent definitive closure.

Material And Method: Defects (7 x 4 cm) were created in the anterior abdominal wall of New Zealand white rabbits and were repaired using an oval-shaped patch of a prosthesis designed by our team (PL-PU99) or a prosthesis of biological origin (Surgisis) of similar dimensions to the defects.