Quantification strategy of absolute chemiluminescence efficiency for systems of luminol with hydrogen peroxide.

An important feature to be determined in mechanistic studies on chemiluminescence (CL) is its quantum efficiency, which can give significant chemical reaction information on the influence of the reactant structures and reaction conditions. However, most of the previous quantitative measurements of luminescence and quantum efficiencies are complex and incomplete. To overcome the inconvenience and underestimated quantum efficiency in each measurement, we report a simple and highly effective strategy to determine the absolute CL quantum efficiencies for three systems of luminol with hydrogen peroxide by means of a spectrometer along with an integrating sphere.