Long-term grazing reduces soil fungal network complexity but enhances plant-soil microbe network connectivity in a semi-arid grassland.

Grazing plays a significant role in shaping both aboveground vegetation and belowground microbial communities in arid and semi-arid grasslands, which in turn affects ecosystem functions and sustainability. Therefore, it was essential to implement effective grazing management practices to preserve ecological balance and support sustainable development in these delicate environments. To optimize the traditional continuous grazing policy, we conducted a 10-year seasonal grazing experiment with five treatments in a typical grassland in northern China: no grazing (NG), continuous summer grazing (CG), and three seasonal grazing treatments (G57 in May and July, G68 in June and August, and G79 in July and September).

Effects of seasonal grazing on plant and soil microbial diversity of typical temperate grassland.

Biodiversity is the decisive factor of grassland ecological function and process. As the most important human use of grassland, grazing inevitably affects the grassland biodiversity. However, comprehensive studies of seasonal grazing on plant and soil bacterial, archaeal and fungal diversity of typical temperate grassland are still lacking.

Impacts of Short-Term Grazing Intensity on the Plant Diversity and Ecosystem Function of Alpine Steppe on the Qinghai-Tibetan Plateau.

Livestock grazing is the primary land use of grasslands worldwide. Grazing has been asserted to alter grassland ecosystem functions, such as productivity, nutrient cycling, and biodiversity conservation. However, few studies have focused on the impact of grazing intensity on the ecosystem multifunctionality (EMF) of alpine grasslands.

Response and contribution of bacterial and archaeal communities to eutrophication in urban river sediments.

Excessive loading of nitrogen (N) and phosphorus (P) that leads to eutrophication mutually interacts with sediment microbial community. To unravel the microbial community structures and interaction networks in the urban river sediments with the disturbance of N and P loadings, we used high-throughput sequencing analysis and ecological co-occurrence network methods to investigate the responses of diversity and community composition of bacteria and archaea and identify the keystone species in river sediments. The alpha-diversity of archaea significantly decreased with the increased total nitrogen (TN), whereas the operational taxonomic unit (OTU) number of bacteria increased with the increase of available phosphorus (AP).

History of petroleum disturbance triggering the depth-resolved assembly process of microbial communities in the vadose zone.

Biogeochemical gradient forms in vadose zone, yet little is known about the assembly processes of microbial communities in this zone under petroleum disturbance. This study collected vadose zone soils at three sites with 0, 5, and 30 years of petroleum contamination to unravel the vertical microbial community successions and their assembly mechanisms. The results showed that petroleum hydrocarbons exhibited higher concentrations at the long-term contaminated site, showing negative impacts on some soil properties, retarding in the surface soils and decreasing along soil depth.

Geo-distribution pattern of microbial carbon cycling genes responsive to petroleum contamination in continental horizontal oilfields.

Contamination significantly affects soil microbial community structures, and the metabolisms of organic contaminants might particularly alter soil carbon cycling by shaping microbial carbon cycling genes. Although numerous studies have discussed the impacts of petroleum contamination on soil bacterial communities and relevant degrading genes, there is no work addressing how soil carbon cycling genes are affected by petroleum contamination. In this study, 77 soil samples were collected from five typical oilfields horizontally located in China to explore the influence of environmental variables and petroleum contamination on microbial carbon cycling genes.

Disturbance, carbon physicochemical structure, and soil microenvironment codetermine soil organic carbon stability in oilfields.

The stability of soil organic carbon (SOC) is crucial for soil quality, fertility, and natural attenuation processes of pollutants. The physicochemical structures of SOC were believed to control its stability, yet has become controversial. Here we hypothesized that disturbance intensity and variations in the soil environment can also influence the SOC stability, and conducted a case study with oil contaminated soils to quantify the contributions to SOC stability of various factors including contamination level, carbon physicochemical structure, and soil properties.

Microbial functional gene patterns related to soil greenhouse gas emissions in oil contaminated areas.

Linking microbial community structure to physiology and ecological processes is a critical focus of microbial ecology. To understand the microbial functional gene patterns related to soil greenhouse gas [carbon dioxide (CO), methane (CH) and nitrous oxide (NO)] emissions under oil contamination, we used functional gene array (GeoChip 5.0) analysis and network methods to investigate the feedback responses of soil microbial functional gene patterns and identify keystone genes in Shengli Oilfield, China.

[Effects of different land-use types on soil organic carbon and its prediction in the mountainous areas in the middle reaches of Lancang River].

Based on the analysis of 210 surface soil samples (0-20 cm) according to field investigation in 2011, soil organic carbon (SOC) content and its distribution under four different land-use types in the middle reaches of Lancang River were studied. Combining with the correlation analysis, relationships among SOC content, land-use type, topographical factors and NDVI were revealed. Furthermore, multiple linear regression and Kriging method were used to predict the SOC contents distribution in the mountainous areas in the middle reaches of Lancang River.

[Effects of road construction on regional vegetation types].

As a regional artificial disturbance component, road exerts great effects on vegetation types, and plays a substantial role in defining vegetation distribution to a certain extent. Aiming at the tropical rainforest degradation and artificial forest expansion in Yunnan Province of Southwest China, this paper analyzed the effects of road network extension on regional vegetation types. In the Province, different classes of roads had different effects on the vegetation types, but no obvious regularity was observed in the effects on the patch areas of different vegetation types due to the great variations of road length and affected distance.