The role of NGX6 and its deletion mutants in the proliferation, adhesion and migration of nasopharyngeal carcinoma 5-8F cells.

Objective: To demonstrate the subcellular localization of nasopharyngeal carcinoma (NPC)-associated gene 6 (NGX6) and its basic structure and function.

Methods: The deletion mutants of NGX6 were constructed by one-step PCR and transfected into NPC cell line 5-8F. The subcellular location of NGX6 or its mutants was detected by immunofluorescence staining of cytoplasm (CYTO) and nuclear protein, and immunoelectron-microscopic analysis.

[Metabolomics and its application in malignant tumors].

Metabolomics is a new science and technology, which it refers to a holistic analytical approach to all the low molecular weight metabolites in an organism or a cell. In this paper, the definition and objective of metabolomics are provided, and the current application of metabolomic research in malignant tumors (diagnosis and therapy) are summarized.

[Expression of c-terminal of Parkin in E. coli and the preparation of antiserum].

Objective: To clone and express 3'-terminal of Parkin gene in E. coli, and prepare its antiserum for further study.

Methods: The glutathion-sulfate-transferase (GST) fusion expression plasmid of 3'-terminal of Parkin gene (937-1959 bp) was constructed and transferred to JM 105.