Spatiotemporal single-cell profiling reveals that invasive and tissue-resident memory donor CD8 T cells drive gastrointestinal acute graft-versus-host disease.

Organ infiltration by donor T cells is critical to the development of acute graft-versus-host disease (aGVHD) in recipients after allogeneic hematopoietic stem cell transplant (allo-HCT). However, deconvoluting the transcriptional programs of newly recruited donor T cells from those of tissue-resident T cells in aGVHD target organs remains a challenge. Here, we combined the serial intravascular staining technique with single-cell RNA sequencing to dissect the tightly connected processes by which donor T cells initially infiltrate tissues and then establish a pathogenic tissue residency program in a rhesus macaque allo-HCT model that develops aGVHD.

Evidence for persistence of the SHIV reservoir early after MHC haploidentical hematopoietic stem cell transplantation.

Allogeneic transplantation (allo-HCT) has led to the cure of HIV in one individual, raising the question of whether transplantation can eradicate the HIV reservoir. To test this, we here present a model of allo-HCT in SHIV-infected, cART-suppressed nonhuman primates. We infect rhesus macaques with SHIV-1157ipd3N4, suppress them with cART, then transplant them using MHC-haploidentical allogeneic donors during continuous cART.

State boards of nursing and the bridge to quality.

The 2003 Institute of Medicine report, Health Professions Education: A Bridge to Quality, delineated a set of core competencies for health care professionals-providing patient-centered care, working in interdisciplinary teams, using evidence-based practice, applying quality improvement processes, and using informatics. The purpose of this study is to examine the extent to which these core competencies have been incorporated in the rules and regulations of state boards of nursing in the United States as required curricular content for professional nursing programs. A research team compiled state boards of nursing regulations related to prelicensure nursing curricula from all 50 states, and content analysis was performed.

From buy-in to integration: melding an interprofessional initiative into academic programs in the health professions.

Improvement in the provision of health care is essential in a complex, diverse and changing system. Studies are beginning to indicate that interprofessional (IP) collaboration can be a means to help address this issue. Institutions that have programs in health professions develop Interprofessional Education (IPE) programs as a way to introduce and nurture the knowledge, skills, and attitudes necessary for IP collaboration.

Development and evaluation of evidence-based nursing (EBN) filters and related databases.

Objectives: Difficulties encountered in the retrieval of evidence-based nursing (EBN) literature and recognition of terminology, research focus, and design differences between evidence-based medicine and nursing led to the realization that nursing needs its own filter strategies for evidence-based practice. This article describes the development and evaluation of filters that facilitate evidence-based nursing searches.

Methods: An inductive, multistep methodology was employed.