Cytotoxicity and stress gene microarray analysis in cadmium-exposed CRL-1439 normal rat liver cells.

Cadmium is a biologically non-essential divalent hazardous metal. Previous studies demonstrated that cadmium toxic effect was caused by reactive oxygen species. Since gene expression is influenced by the presence of these reactive oxygen species, the association between metal intoxication and gene expression has recently become a major focus of research.

Comparative evaluation of cytotoxicity of cadmium in rat liver cells cultured in serum-containing medium and commercially available serum-free medium.

Cadmium (Cd) is an industrial pollutant and carcinogenic metal. Most in vitro Cd toxicity studies have been carried out in various cell lines cultured in 10% fetal bovine serum (FBS) containing medium. In this report, we compared the toxic effect of Cd (0-300 microM) on cell growth, total RNA, total proteins, and antioxidant enzymes in rat normal liver cells cultured in medium with 10% FBS or commercially available serum-free medium for 4 or 8 hours.

Mechanism of DNA damage by cadmium and interplay of antioxidant enzymes and agents.

Cadmium is an environmental toxicant, which causes cancer in different organs. It was found that it damages DNA in the various tissues and cultured cell lines. To investigate the mechanism of DNA damage, we have studied the effect of cadmium-induced DNA damage in plasmid pBR322 DNA, and the possible ameliorative effects of antioxidative agents under in vitro conditions.