The attenuation of doxorubicin-induced testicular toxicity with improved testicular histoarchitecture of mice by the bioactive compounds in leaves: Experimental and computational studies.

Doxorubicin, as an antibiotic causes toxicity in human tissues through the generation of oxidant species; however, (Solanaceae) is ethnopharmacologically and scientifically reported to possess antidotal activities. This study was designed to validate the antidotal potency of the plant's bioactive compounds on rats' testes following induction with doxorubicin through the evaluation of oxidative stress markers, lipid peroxidation indices, testes' histological sections, and profiling of the plant's bioactive compounds against some proteins. The collection and preparation of the plant extract, testicular toxicity induction, seminal analysis, assay of testosterone and oxidative stress markers, lipid peroxidation profiling, histomorphological studies, retrieval of catalase, superoxide dismutase, and glutathione peroxidase from PDB, GC-MS, ADME, and docking analyses followed standard protocols.

Betulinic and ursolic acids from Nauclea latifolia roots mediate their antimalarial activities through docking with PfEMP-1 and PfPKG proteins.

Background: Chemotherapies target the PfEMP-1 and PfPKG proteins in Plasmodium falciparum, the parasite that causes malaria, in an effort to prevent the disease's high fatality rate. This work identified the phytochemical components of Nauclea latifolia roots and docked the chemical compounds against target proteins, and examined the in vivo antiplasmodial effect of the roots on Plasmodium berghei-infected mice.

Methods: Standard protocols were followed for the collection of the plant's roots, cleaning, and drying of the roots, extraction and fraction preparation, assessment of the in vivo antiplasmodial activity, retrieval of the PfEMP-1 and PfPKG proteins, GCMS, ADME, and docking studies, chromatographic techniques were employed to separate the residual fraction's components, and the Swis-ADME program made it possible to estimate the drug's likeness and pharmacokinetic properties.

Toxicological evaluation of a polyherbal formulation on testicular function and gonadal histomorphology in exposed Wistar rats.

Introduction: Dr Iguedo Goko Cleanser® is a herbal formulation (HF) widely marketed in southern Nigeria and purported to be very efficacious for the management of various diseases including giardiasis, toilet infections, hypertension, diabetes, ulcer, impotence, low libido, low sperm count amongst others. Medicinal plants reportedly produce an array of adverse reactions capable of inducing harmful conditions, including death.

Aim: This study evaluated the subchronic toxicity concern of HF on testicular function and gonadal histoarchitecture in Wistar rats.

Antiplasmodial activity and cytotoxicity of ethanol extract of root.

Objective: root decoction that has been traditionally used for the treatment of malaria by various tribes in Nigeria, was evaluated for antimalarial potential against malaria parasites using and models.

Materials And Methods: The root extract of was investigated for antimalarial activity against in mice using rodent malaria models; suppressive, prophylactic and curative tests and antiplasmodial activity against chloroquine-sensitive (Pf 3D7) and resistant (Pf INDO) strains of using SYBR green assay method. Median lethal dose and cytotoxic activity against HeLa and HEKS cells were assessed and phytochemical screening was also carried out using standard procedures.

Hepatoprotective activity of the extract of stem against paracetamol-induced liver injury.

Objective: Pellegr (Flacourtiaceae) has been traditionally used by the Ibibios of Southern Nigeria to treat stomach ulcer, malaria and other inflammatory diseases and Yorubas of western Nigeria as an antidote. This study evaluates the hepatoprotective properties of the ethanol extract of the plant stem.

Materials And Methods: The hepatoprotective effect of the extract of the stem of the plant (200-600 mg/kg) was evaluated by the assay of liver function parameters, namely total and direct bilirubin, serum protein and albumin, total cholesterol, alanine aminotransaminase (ALT), aspartate aminotransaminase (AST), and alkaline phosphatase activities (ALP), antioxidant enzymes like superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), reduced glutathione (GSH) and histopathological study of the liver.

Hepatoprotective activity of Mammea africana ethanol stem bark extract.

Objective: The stem bark of Mammea africana Sabine (Guttiferae), (M. africana) a common plant that has been traditionally used to treat various diseases and ailments was evaluated for hepatoprotective potentials against paracetamol-induced liver injury in rats.

Materials And Methods: The hepatoprotective effect of the stem bark extract (30-90 mg/kg) was evaluated by the assay of liver function parameters, namely total and direct bilirubin, serum protein and albumin, total cholesterol, alanine aminotransaminase (ALT), aspartate aminotransaminase (AST), and alkaline phosphatase activities (ALP), antioxidant enzymes: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH) and histopathological study of the liver.

Cellular antioxidative, cytotoxic, and antileishmanial activities of Homalium letestui.

Objective: Homalium letestui Pellegr (Flacourtiaceae) is used in traditional medicine in parts of Nigeria for the treatment of malaria, ulcer, and inflammatory diseases and as an aphrodisiac. This investigation was aimed to evaluate the cytotoxic, immunomodulatory, and antileishmanial properties of stem extract and fractions of Homalium letestui (H. letestui).

Antidiarrhoeal and antiulcer activities of Hippocratea africana root extract.

The effect of ethanol root extract of H. africana on experimentally induced diarrhoea and ulcer was studied in rodents. The extract (200-600 mg/kg) considerably inhibited castor oil induced diarrhoea, small intestine transit time and castor oil induced fluid accumulation as well as indomethacin and ethanol induced ulcer models.