Diastolic coronary resistance and capacitance are independent of the duration of diastole.

Systolic myocardial contraction may produce changes in coronary resistance and capacitance that persist throughout a normal diastole. In addition, coronary resistance and capacitance as determined in the arrested heart may not accurately describe normal diastolic behavior. To evaluate these possibilities, an identification method capable of characterizing the input impedance of the coronary circulation in as little as 150 ms was developed.

Tone-dependent waterfall behavior during venous pressure elevation in isolated canine hearts.

We examined the "vascular waterfall" hypothesis, which proposes that coronary flow is unaffected by elevations in outflow pressure until the latter reaches a critical threshold level, in 29 isolated canine hearts. In fibrillating hearts vasodilated with adenosine or carbocromen, coronary flow and the coronary pressure-flow relation were not affected by changes in great cardiac vein pressure (PGCV) below a threshold value of 11 +/- 0.9 (mean +/- SEM) mm Hg.

Effects of barium-induced cardiac contraction on large- and small-vessel intramyocardial blood volume.

We have measured the effects of barium-induced cardiac contraction on the intramyocardial blood volume content of all vessels and have independently measured the blood volume content of vessels with a diameter greater than 100 microns in rat myocardium. Measurements of total intramyocardial blood volume were made by using [125I]albumin as a plasma marker and technetium-99m as a red blood cell marker. In one group of rats (n = 8), diastolic arrest was induced by an intravenous injection of KCl; in a second group (n = 8), systolic arrest was induced by an intravenous injection of BaCl2.

Characterization of fourteen strains of Neisseria gonorrhoeae: structural analyses and serum reactivities.

Resistance to normal human serum (NHS) killing in Neisseria gonorrhoeae has been associated with particular types of Protein I (PI) and lipopolysaccharide (LPS), but many exceptions exist, and the role of these structures in determining serum reactivities remains controversial. In reality, the response of the gonococcus to NHS is probably governed by several parameters involving a number of outer-membrane (OM) components. We surveyed the serum reactivities of 14 strains of N.

Comparison of two serum bactericidal assays for Neisseria gonorrhoeae.

Pooled normal human serum killing of 14 strains of Neisseria gonorrhoeae was assessed by dilution plate and microtiter methods. In both assays, the strains presented a spectrum of sensitivity to the serum. In the dilution plate assay, results with two different concentrations of human serum were similar for most, but not all of the strains tested.

Apocrine adenoma of the breast: report of a case with investigation of lectin binding patterns in apocrine breast lesions.

A 19-year-old female presented with a left breast mass clinically felt to be a fibroadenoma. Pathologically, it was found to be a pure apocrine adenoma of the breast. This is an extremely rate entity, with this case being only the third to be reported.

Characterization of reverse transcriptase from feline immunodeficiency virus.

Reverse transcriptase has been purified from feline immunodeficiency virus (FIV) by DEAE-cellulose and phosphocellulose chromatography. The purified enzyme consists of a single protein with a Mr of 67,000. When proteolysis is not minimized during purification, a fragment of Mr 54,000 is also observed.

Acute human immunodeficiency virus infection temporally associated with rhabdomyolysis, acute renal failure, and nephrosis.

A previously healthy 29-year-old homosexual man presented with a 4-day history of fever, malaise, sore throat, and bleeding gums. Rhabdomyolysis, acute renal failure, and nephrotic range proteinuria were also present. The patient was found to have acute human immunodeficiency virus (HIV) infection confirmed by the presence of HIV antigen in his serum and subsequent evolution of an HIV antibody profile typical of acute seroconversion.

Combined true thymic hyperplasia and lymphoid hyperplasia in Graves' disease.

True thymic hyperplasia (enlarged gland composed of histologically unremarkable cortical and medullary parenchyma) and lymphoid hyperplasia (medullary lymphoid follicles in the clinical setting of autoimmunity) usually develop as independent pathologic processes. We reviewed the clinical features and gross and microscopic pathology of 2 hyperthyroid patients with features of both thymic hyperplasia and lymphoid hyperplasia. The diagnosis of thymic hyperplasia was supported by thymic weights greater than two standard deviations above the mean weight for age and histologic evidence of expanded cortical and medullary parenchyma.

Identification and characterization of peptidoglycan-associated proteins in Neisseria gonorrhoeae.

The principal proteins associated with Neisseria gonorrhoeae peptidoglycan (PG), as identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, are the following: two proteins at approximately 90 kilodaltons (kDa), single major species at both 60 and 44 kDa, a 34- to 36-kDa protein, and three proteins between 28 and 32 kDa. A protein analogous to Escherichia coli Braun lipoprotein was not detected with gonococcal cell wall preparations. The identity of the PG-associated proteins was confirmed immunologically with antibody generated against purified cell walls.

Multiple arteriovenous malformations of the small intestine in a patient with protein S deficiency.

The authors report a case of arteriovenous malformations (AVMs) of the small intestine in a young patient with protein S deficiency. These disorders have not been previously reported to occur together. Protein S deficiency may cause thromboses in unusual sites, including the mesenteric veins.

Complement binding on serum-sensitive and serum-resistant transformants of Neisseria gonorrhoeae: effect of presensitization with a non-bactericidal monoclonal antibody.

The binding of C3 and C9 on serum sensitive (FA635) and serum resistant (FA638) transformants of serum sensitive Neisseria gonorrhoeae strain F62 was examined. Previous studies showed that these transformants have Protein IAs which are minimally different by proteinase K cleavage and primary structural and peptide mapping and bear LPS which vary slightly on SDS-PAGE. Binding of C3 and C9 on FA635 exceeded binding on FA638 in NHS and in adsorbed NHS.

Topographical alterations in proteins I of Neisseria gonorrhoeae correlated with lipooligosaccharide variation.

Four transformant strains of Neisseria gonorrhoeae were generated, two of which (WS3 and WS5) had protein I subclass A (P.IA) and two which (WS2 and WS4) had protein I subclass B (P.IB).

Export and intercellular transfer of DNA via membrane blebs of Neisseria gonorrhoeae.

Naturally elaborated membrane bleb material is frequently observed in cultures of Neisseria gonorrhoeae. This material was purified and analyzed for protein, lipopolysaccharide, and nucleic acid content. The electrophoretic protein profiles of two bleb-rich fractions, called BI and BII, were distinct, with only BII containing lipopolysaccharide and outer membrane proteins I and III.

Mathematically directed single-cut osteotomy for correction of tibial malunion.

In 1986 and 1987, four patients with tibial malunion involving both angular and torsional misalignment underwent surgical correction by a single-cut technique based on a mathematical model of long bone deformity. The technique involved a single surgical cut, followed by rotation of the two fragments along the osteotomy plane. The orientation of the osteotomy is based on a computer-assisted design.

Myoid cell differentiation in true thymic hyperplasia and lymphoid hyperplasia.

Four cases of thymic hyperplasia (TH) and/or lymphoid hyperplasia (LH) were investigated immunohistochemically for evidence of myoid cell (MC) and epithelial cell (EC) differentiation using antibodies to myoglobin, desmin, and cytokeratin. Although MCs were identified in all the cases, the most impressive perifollicular proliferation of ECs and MCs was seen in the hyperthyroid patient with TH/LH, suggesting that these cells have a role in immune function. The unusual combination of LH and TH in this patient demonstrates that exceptional cases can show overlapping features of what are otherwise considered to be separate thymic lesions.

Purification of outer membrane proteins of the gram-negative bacterium Neisseria gonorrhoeae.

A system of protein purification, using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electroblotting, that results in purified outer membrane proteins of the gram-negative bacterium Neisseria gonorrhoeae is described. The proteins, which ranged in apparent molecular mass from approximately 31,000 to approximately 92,000 Da, were located by naphthol blue black staining, eluted from nitrocellulose membranes using 88% formic acid, and precipitated by the addition of concentrated ammonium hydroxide. Up to 65% of the original protein present was recovered by this procedure.

Massive thymic hyperplasia with myoid cell differentiation.

A 12-year-old boy with massive true thymic hyperplasia presented with respiratory distress and dysphagia. The thymus weighed 245 g and demonstrated normal cortical and medullary components histologically. The findings in this case were compared with the clinical and pathologic features of seven previously published cases of massive hyperplasia and with cases of mild or "borderline" hyperplasia.

A 37-pound scrotal leiomyosarcoma: a case report and literature review.

We report a case of a 37-pound scrotal leiomyosarcoma. The literature is reviewed, and the presentation, diagnosis, treatment and survival of patients with scrotal leiomyosarcoma are discussed.