P-Doping Modulated RuIr Nanoparticles Anchored on Co/N/C Catalysts with Improved Alkaline Hydrogen Evolution Activity and Stability.

Achieving efficient and stable hydrogen evolution reactions in alkaline conditions is crucial for hydrogen production. In this study, a RuIr/CoNC-P catalyst featuring RuIr alloys alongside P-doping and CoNx sites is developed. RuIr alloying optimizes the electronic structure between Ru and Ir, promoting electron transfer from Ru to Ir.

Triple C-H Activation/Annulation: In Situ Construction of Fluorescent Peptides.

Herein, we report a Rh(III)-catalyzed triple C-H activation-annulation of Phe-based peptides with alkynes for the preparation of fluorescent peptides. The robustness of this protocol is reflected by a broad substrate scope, high atom- and step-economy, and excellent chemo- and site-selectivity. An in situ generated polycyclic aromatic hydrocarbon carbocation as a fluorophore exhibits good fluorescence properties (maximum emission wavelength up to 628 nm) and low cell cytotoxicity.

Depletion of Tregs from CD4 CAR-T cells enhances the tumoricidal effect of CD8 CAR-T cells in anti-CD19 CAR-T therapy.

Chimeric antigen receptor T (CAR-T) cell therapy, which targets CD19 for hematological malignancies, represents a breakthrough in cancer immunotherapy. However, some patients may develop resistance to CAR-T treatment, underscoring the importance of optimizing CAR-T design to enhance responsiveness. Here, we investigated the impact of different subpopulations in anti-CD19 CAR-T cells on the tumoricidal effect.

Super signal-enhancement biosensing platform for precise target recognition based on rolling circle-hybridization chain dual linear cascade amplification technology.

This paper presents a self-powered biosensing platform based on graphdiyne@Au (2D GDY@Au) nanoparticles and rolling circle-hybridization chain (RC-HC) dual linear cascade amplification technology, which significantly enhances target recognition and signal amplification efficiency for miRNA-141. Specifically, the target on bioanode outputs a large amount of single-stranded DNA (T1) through the strand displacement amplification (SDA) mechanism. This efficient target recycling process triggers RC-HC dual linear cascade reaction.

Palmitoyl copper peptide and acetyl tyrosine complex enhances melanin production in both A375 and B16 cell lines.

Copper peptide, a low molecular weight peptide composed of glycyl-L-histidyl-l-lysine-copper, possesses anti-aging, anti-inflammatory, and wound healing properties. In this study, we investigated the effects of a combination agent CP-AcT, composed of palmitoyl copper peptide (pal-GHK-Cu) and acetyl tyrosine (N-Acetyl-l-tyrosine), on melanin production in the human malignant melanoma cell line A375 and the mouse melanoma cell line B16. Firstly, the cytotoxicity of CP-AcT at various concentrations (0-8 μg/mL) on HaCat, HFF, A375, and B16 cells was evaluated.

Coarse-Grained Simulation Study of the Association of Selected Dipeptides.

The association of 55 dipeptides extracted from aggregation-prone regions of selected proteins was studied by means of multiplexed replica-exchange molecular dynamics simulations with the coarse-grained UNRES model of polypeptide chains. Each simulation was carried out with 320 dipeptide molecules in a periodic box at 0.24 mol/dm concentration, in the 260-370 K temperature range.

Intranasal oxytocin alleviates postsurgical pain and comorbid anxiety in mice: Participation of BK(Ca) channels in the hippocampus.

The affective dimension in postsurgical pain is still poorly understood. Since neuropeptide oxytocin (OXT) has been implicated in a broad spectrum of pain and negative emotion, we investigated the potential therapeutic effect of intranasal OXT on postsurgical pain and associated anxiety in a mice model of plantar incision. The role of large conductance Ca(2+)-activated K(+) (BK(Ca)) channels was explored by using behavioral pharmacology experiments.

Virtual Reality-based Infrared Pupillometry (VIP) for long COVID.

Objective: To evaluate the use of virtual reality-based infrared pupillometry (VIP) to detect individuals suffering long COVID.

Design: Prospective, case-control cross-sectional study.

Participants: Participants aged 20-60 were recruited from a community eye screening programme.

Molecular characterization of ST15 carbapenem-resistant Klebsiella pneumoniae isolated in a single patient.

Background: The carbapenem-resistant Klebsiella pneumoniae (CRKP) poses a serious threat to antibiotic applicability and public health. During treatment, K. pneumoniae (KP) frequently exhibits shifts in drug-resistant phenotypes, complicating clinical treatment as it transitions from sensitivity to resistance.

Self-assembled nano delivery system of fenugreek polysaccharides: Effects on curcumin bioavailability and molecular mechanisms.

Self-assembly of fenugreek polysaccharides FS60 (a natural macromolecular material) with curcuminoid has been proved to improve curcumin (Cur) water dispersion in preliminary studies. This study further explored the effect of FS60 on Cur bioavailability in vivo to assess the significance of this delivery method. In this study, we optimized the formulation parameters of FS60-curcuminoid aggregates (FC) and studied their effects on Cur pharmacokinetics in rats.

CaNAC76 enhances lignin content and cold resistance in pepper by regulating CaCAD1.

Low temperature restricts the growth, development, and yield of peppers, significantly limiting the development of the pepper industry. NAC (NAM, ATAF1/2, and CUC2) transcription factors (TFs) are implicated in plant responses to cold stress, but their specific mechanisms in peppers are unclear. In this study, we isolated a cold-induced NAC transcription factor, CaNAC76, from pepper (Capsicum annuum L.

Retrospective analysis of the clinical outcomes of paraspinal soft-tissue sarcoma.

Background Context: Due to the rarity and heterogeneity, paraspinal soft-tissue sarcomas represent a unique and challenging clinical entity for musculoskeletal oncologist. Reports on the treatment of paraspinal soft-tissue sarcoma are limited and the best treatment modality for this rare sarcoma type has not yet been well defined.

Purpose: To introduce a modified classification of paraspinal sarcoma, review the outcome of patients treating in our center and determine the effective treatment for paraspinal soft-tissue sarcoma.

T1WI Radiomics Analysis of Anterior Scalene Muscle: A Preliminary Application in Neurogenic Thoracic Outlet Syndrome.

Aim: This study aimed to analyze the differences in radiomic features of the anterior scalene muscle and evaluate the diagnostic performance of MRI-based radiomics model for neurogenic thoracic outlet syndrome (NTOS).

Materials And Methods: Imaging data of patients with NTOS who underwent preoperative brachial plexus magnetic resonance neurography were collected and were randomly divided into training and test groups. The anterior scalene muscle area was sliced in the T1WI sequence as the region of interest for the extraction of radiomics features.

The Value of Whole-Volume Radiomics Machine Learning Model Based on Multiparametric MRI in Predicting Triple-Negative Breast Cancer.

Objective: This study aimed to investigate the value of radiomics analysis in the precise diagnosis of triple-negative breast cancer (TNBC) based on breast dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) and apparent diffusion coefficient (ADC) maps.

Methods: This retrospective study included 326 patients with pathologically proven breast cancer (TNBC: 129, non-TNBC: 197). The lesions were segmented using the ITK-SNAP software, and whole-volume radiomics features were extracted using a radiomics platform.

Genetic and microenvironmental evolution of colorectal liver metastases under chemotherapy.

Drug resistance limits the efficacy of chemotherapy for colorectal cancer liver metastasis (CRLM). However, the evolution of CRLM during drug treatment remains poorly elucidated. Multi-omics and treatment response data from 115 samples of 49 patients with CRLM undergoing bevacizumab (BVZ)-based chemotherapy show little difference in genomic alterations in 92% of cases, while remarkable differences are observed at the transcriptomic level.

Changes in selenium bioavailability in selenium-enriched paddy soils induced by different water management and organic amendments.

Combined effects of water management and agricultural organic waste return on selenium (Se) bioavailability and mechanisms in Se-enriched paddy soils remain unclear. We investigated the effects of continuous flooding (CF) and alternating wet and dry (AWD), two types (cotton straw biochar [BC] and sheep manure [SM]) and concentrations (10 and 50 g·kg) of organic amendments on soil Se bioavailability, bacterial community structure in naturally Se-enriched soils (1.69 mg·kg).

Atmospheric deposition of pollutants at three altitudes on Mount Emei, Sichuan Basin, southwestern China.

Comparing the atmospheric deposition chemistry between above and below the planetary boundary layer (PBL) may help understand the impacts from inter-regional air pollutant transport and local emissions to air pollutant deposition. In this study, we monitored ions, soluble and insoluble potentially toxic elements (PTEs), and dissolved organic nitrogen and carbon at the base (M-base: 551 m asl), middle (M-middle: 2400 m asl), and summit (M-summit: 3077 m asl) on Mount Emei. The annual volume-weighted mean (VWM) concentrations of all measured components were substantially higher at M-base than at M-summit, except for Na, Cl, and NO as Na and Cl at M-summit may be largely from ocean and NO to NO transformation may be faster at M-base.

Industrial hemp (Cannabis sativa L.) adapts to cadmium stress by reshaping rhizosphere fungal community.

Increasing evidence indicates that plants under environmental stress can actively seek the help of microbes ('cry-for-help' hypothesis). However, empirical evidence underlying this strategy is limited under metal-stress conditions. Here, we employed integrated microbial community profiling in cadmium (Cd) polluted soil and culture-based methods to investigate the three-way interactions between the industrial hemp (Cannabis Sativa L.

Co/CoO@NC-CNTs modified separator of Li-S battery achieving the synergistic effect of adsorption-directional migration-catalysis via built-in electric field.

The shuttle effect of lithium polysulfides (LiPSs) and sluggish sulfur conversion kinetics have seriously hindered the commercial application of lithium-sulfur (Li-S) batteries. Currently, the adsorption and catalysis processes are emphasized; however, the diffusion process is often neglected. The delayed diffusion of the adsorbed LiPSs significantly reduce battery performance.

Sequential activation of osteogenic microenvironment via composite peptide-modified microfluidic microspheres for promoting bone regeneration.

The osteogenic microenvironment (OME) significantly influences bone repair; however, reproducing its dynamic activation and repair processes remains challenging. In this study, we designed injectable porous microspheres modified with composite peptides to investigate cascade alterations in OME and their underlying mechanisms. Poly -lactic acid microfluidic microspheres underwent surface modifications through alkaline hydrolysis treatment, involving heterogeneous grafting of bovine serum albumin nanoparticles with stem cell-homing peptides (BNP@SKP) and BMP-2 mimicking peptides (P24), respectively.