Neutrophil membrane-coated nanoparticles for targeted delivery of toll-like receptor 4 siRNA ameliorate LPS-induced acute lung injury.

Pulmonary delivery of small interfering RNAs (siRNAs) is an effective treatment for acute lung injury (ALI), which can modulate the expression of pro-inflammatory cytokines and alleviate the symptoms of ALI. However, the rapid degradation of siRNA in vivo and its limited ability to target and validate cells are important challenges it faces in clinical practice. In this work, we developed neutrophil membrane-coated Poly (lactic-co-glycolic acid) nanoparticles loaded with TLR4 siRNA (si-TLR4) (Neutrophil-NP-TLR4), which can target both inflammatory and macrophage cells to alleviate the pulmonary inflammation in lipopolysaccharide (LPS)-induced ALI mice.

Knockdown of VDAC1 alleviates the cognitive dysfunction secondary to sepsis-associated encephalopathy.

Sepsis-associated encephalopathy (SAE) is a serious and diffuse cerebral dysregulation with a high morbidity and mortality caused by sepsis. Mitophagy plays an important role in SAE, and microglial phagocytosis of apoptotic cells (efferocytosis) is the core of the brain regenerative response. Voltage dependent anion channel (VDAC1) is an important regulator of mitophagy.

The long-term environmental risks from the aging of organochlorine pesticide lindane.

Although increased contact time (aging) of pesticides in the soil decreases their bioavailability, this does not mean that the bound residues formed during the aging process pose fewer risk to the soil environment. Here the earthworm Eisenia fetida was exposed to organochlorine pesticide lindane in soil under different durations of lindane aging and exposure. The results of de novo RNA sequencing followed by molecular and biochemical validations demonstrated the aged lindane showed a different tendency to disrupt acetylcholine (ACh) transmission with the effects of fresh lindane to gamma-aminobutyric acid.

A sensitive optical-based test method for the locomotor activity of earthworms.

The outdated test methodologies for terrestrial animals have limited the progress of soil ecotoxicology to some extent. To improve the behavioral testing of earthworms, a terrestrial model animal, a sensitive optical-based method for detecting locomotor activity was established. The method measured the fine quantified position offsets of each earthworm in place of a conventional overall response rate, which provided the feasibility for accurate analyses and comparisons.

Bioaccumulation, distribution and elimination of lindane in Eisenia foetida: The aging effect.

Soil aging will influence the chemical speciation of pesticides, thus affecting the uptake route to be bioavailable to the organism. So far, studies on the possible effects of the uptake route on the distribution and elimination of pesticides in the organism that also considers effects of aging are limited. In our study, Eisenia fetida was exposed to 4.

Seasonal variation in air particulate matter (PM10) exposure-induced ischemia-like injuries in the rat brain.

Epidemiological studies imply a significantly positive association between particulate matter (PM) level and ischemic stroke hospitalization. However, considering that PM10 is highly heterogeneous and varies with season within the same location, existing experimental evidence remained low. In the present study, we first treated Wistar rats with PM10 samples collected from different seasons in Taiyuan, a typically coal-burning city of China, and determined ischemia-related markers in the cortex.