A molecule capturer analysis system for visual determination of avian pathogenic Escherichia coli serotype O78 using a lateral flow assay.

A method for rapid and accurate determination of avian pathogenic Escherichia coli serotype O78 (APEC O78) by the gold nanoparticle-labeled lateral flow strip method, entitled molecule capturer analysis system (MCAS), is described. Target virulence-associated gene of APEC O78 is adopted as the analyte. After pre-amplification with the designed functional primer set, numerous new-formed amplicons are simultaneously labeled with fluorescein isothiocyanate (FITC) and digoxin.

Acetate metabolic requirement of avian pathogenic Escherichia coli promotes its intracellular proliferation within macrophage.

Avian pathogenic Escherichia coli (APEC) is a facultative intracellular pathogen, and intracellular persistence in macrophages is essential for APEC extraintestinal dissemination. Until now, there is still no systematic interpretation of APEC intracellular proliferation. Intracellular survival factors, especially involved in pathometabolism, need to be further revealed.

Comparative genomic analysis of 127 Escherichia coli strains isolated from domestic animals with diarrhea in China.

Background: Escherichia coli is an important pathogen that causes diarrhea in both humans and animals. To determine the relationships between putative virulence factors and pathotypes or host taxa, many molecular studies on diarrhea-associated E. coli have been reported.

Isolation and characterization of a broad-spectrum phage of multiple drug resistant Salmonella and its therapeutic utility in mice.

Salmonella are causes of livestock, poultry, and other animal diseases but they also have the potential to infect people. Currently, antibiotics are the first choice for treatment of Salmonella infections. Thus, the utility of phage has become the research focus for scientists for several reasons.

Lewis Acidity and Basicity of Mixed Chlorometallate Ionic Liquids: Investigations from Surface Analysis and Fukui Function.

Mixed chlorometallate ionic liquids (ILs) have been regarded as potential solvents, catalysts, and reagents for many organic processes. The acidity and basicity of these ILs were correlated with theoretically estimated parameters such as electrostatic surface potential maxima and minima, average local surface ionization energy, and Fukui and dual descriptor functions. The introduction of metal chloride into the anions would influence the acidity/basicity of ILs by withdrawing the electron density from the cationic counterpart.

Prediction of H NMR chemical shifts for ionic liquids: strategy and application of a relative reference standard.

The computational predictions of H NMR chemical shifts for ionic liquids were investigated. To calculate the chemical shifts more accurately, the approach of relative reference standard (RRS) was proposed. This straightforward computational technique uses organic compounds and ionic liquids that are similar to the studied ionic liquids as standards.

A Novel PhoP/PhoQ Regulation Pathway Modulates the Survival of Extraintestinal Pathogenic in Macrophages.

The extraintestinal pathogenic (ExPEC) is a typical facultative intracellular bacterial pathogen. Sensing the environmental stimuli and undertaking adaptive change are crucial for ExPEC to successfully colonize in specific extraintestinal niches. The previous studies show that pathogens exploit two-component systems (TCSs) in response to the host environments during its infection.

Removal of selenate from water by zerovalent iron.

Zerovalent iron (ZVI) has been widely used in the removal of environmental contaminants from water. In this study, ZVI was used to remove selenate [Se(VI)] at a level of 1000 microg L(-1) in the presence of varying concentrations of Cl-, SO(2-)4, NO(-)3, HCO(-)3, and PO(3-)4. Results showed that Se(VI) was rapidly removed during the corrosion of ZVI to iron oxyhydroxides (Fe(OH)).

Selenate reduction in river water by Citerobacter freundii isolated from a selenium-contaminated sediment.

Bacterial reduction of selenate [Se(VI)] to insoluble elemental Se [Se(0)] is an important remedial technology to remove selenium (Se) from Se-impacted water. Citerobacter freundii, a Se(VI) reducer, isolated from a Se-contaminated sediment was assessed for its ability to reduce Se(VI) in a mineral culture medium and natural river water in a series of laboratory batch experiments. The results showed that a combination of yeast extract and glucose used in the culture medium was more effective than yeast extract alone, yeast extract plus sodium acetate, and yeast extract plus sodium lactate for reduction of Se(VI) to Se(0) by C.