Assessment of a 1H high-resolution magic angle spinning NMR spectroscopy procedure for free sugars quantification in intact plant tissue.

In most plants, sucrose is the primary product of photosynthesis, the transport form of assimilated carbon, and also one of the main factors determining sweetness in fresh fruits. Traditional methods for sugar quantification (mainly sucrose, glucose and fructose) require obtaining crude plant extracts, which sometimes involve substantial sample manipulation, making the process time-consuming and increasing the risk of sample degradation. Here, we describe and validate a fast method to determine sugar content in intact plant tissue by using high-resolution magic angle spinning nuclear magnetic resonance spectroscopy (HR-MAS NMR).

Influence of the spinning rate in the HR-MAS pattern of mobile lipids in C6 glioma cells and in artificial oil bodies.

Object: To evaluate how spinning rate affects mobile lipid (ML) resonances visibility in HR-MAS spectra of C6 glioma cells and artificial oil bodies (AOB), as models of cytosolic lipid droplets.

Materials And Methods: Using C6 cells and AOB of two different sizes, 780 ± 580 and 240 ± 293 nm, as models, we acquired HR-MAS pulse and acquire spectra at different spinning rates between 500 and 15,000 Hz, all at 37 °C.

Results: Sample spinning at 15,000 Hz increased by 2.