[Research advance of solid-phase microextraction based on covalent organic framework materials].

Solid-phase microextraction (SPME) is a fast and simple sample preparation technique that enables the enrichment of analytes, and it is used in combination with other detection techniques to provide accurate and sensitive analytical methods. SPME is widely used in environmental monitoring, food safety, life analysis, biomedicine, and other applications. The extractive coating is the core of the SPME technique, and the properties of the extractive coating greatly influence extraction selectivity and efficiency, as well as the enrichment effect.

Genome-wide analysis of Class III peroxidase (PRX) family core genes and functional mechanism of GhPRXR1-A for seed development in Gossypium hirsutum.

Class III peroxidases (PRXs) play critical roles in plant growth and development by oxidizing various substrates with HO. Although many PRXs have been identified and their roles in biotic and abiotic stress responses have extensively investigated in plants. However, functional mechanisms of PRXs in seed development remain poorly understood.

Prevalence and risk factors of sarcopenia in patients with stroke: a systematic review and meta-analysis.

Stroke is the second leading cause of death and the third-leading cause of disability in the world. The skeletal muscles play a key role in disability following stroke. Although many studies have reported the prevalence and risk factors of sarcopenia in patients with stroke, the results have not been synthesized.

Prevalence and Risk Factors of Poststroke Cognitive Impairment: A Systematic Review and Meta-Analysis.

Background: Stroke is a common disease that poses a significant threat to human health. Approximately one-third of stroke patients experience poststroke cognitive impairment (PSCI), which severely impacts their quality of life and survival time. Although the prevalence and risk factors for PSCI have been widely reported, these results have not been synthesized.

Extraction performance of SiO@COF before and after carbonization and online SPE-HPLC-DAD methods for tetrabromobisphenol A derivatives and phthalates.

The property of organic materials can be regulated by a facile carbonization way. In-situ growth of covalent organic framework on silica (SiO@COF) was achieved by room temperature synthesis, then SiO@COF was carbonized to get another material, called SiO@C-COF. The materials were characterized by SEM, BET, XPS, contact angle test and element analysis.

Defect Engineering in CuS Nanoflowers Enables Low-Overpotential and Long-Cycle-Life of Lithium-Oxygen Batteries.

The defect engineering is essential for the development of efficient cathode catalysts for lithium-oxygen batteries. Herein, CuS nanoflowers are fabricated by microwave hydrothermal method. Through theoretical and experimental analysis, the S vacancies are observed, which result in augmented charge around Cu, improved adsorption of LiO and reduced overpotential.

Integrative analyses of long and short-read RNA sequencing reveal the spliced isoform regulatory network of seedling growth dynamics in upland cotton.

The polyploid genome of cotton has significantly increased the transcript complexity. Recent advances in full-length transcript sequencing are now widely used to characterize the complete landscape of transcriptional events. Such studies in cotton can help us to explore the genetic mechanisms of the cotton seedling growth.

Deciphering the dynamic expression network of fiber elongation and the functional role of the GhTUB5 gene for fiber length in cotton based on an introgression population of upland cotton.

Introduction: Interspecific introgression between Gossypium hirsutum and G. barbadense allows breeding cotton with outstanding fiber length (FL). However, the dynamic gene regulatory network of FL-related genes has not been characterized, and the functional mechanism through which the hub gene GhTUB5 mediates fiber elongation has yet to be determined.

Ionic liquid-functionalized covalent organic frameworks on the surface of silica for online solid-phase extraction.

A covalent organic framework (COF) was gown on porous silica with 1,3,5-tri(4-aminophenyl)benzene and 2,5-divinyl-1,4-phenyldiformaldehyde as monomers, and two ionic liquids were grafted to COF by a click reaction. The materials before and after the modification of ionic liquids were separately packed into solid-phase extraction columns (10 × 4.6 mm, i.

Intrinsic capacity decline as a predictor of functional disability in the elderly: A systematic review and meta-analysis.

Objectives: To examine the predictive value of intrinsic capacity decline on functional disability among the elderly.

Design: Meta-analysis.

Methods: PubMed, EMBASE, Web of Science, The Cochrane Library, Wanfang Database, China Knowledge Resource Integrated Database (CNKI), Weipu Database (VIP), and Chinese Biomedical Database (CBM) were searched for relevant studies published from the inception until June 1, 2024.

The prevalence and characteristics of alexithymia in stroke patients: a systematic review and meta-analysis.

Background: Previous studies have indicated the potential occurrence of alexithymia among stroke patients, yet the prevalence of alexithymia in this population remains disparate across different investigations without a synthesized overview.

Aim: To systematically evaluate the prevalence and characteristics of alexithymia in stroke patients.

Methods: A systematic review and meta-analysis was conducted following the PRISMA guidelines.

Structural insights into curdlan degradation via a glycoside hydrolase containing a disruptive carbohydrate-binding module.

Background: Degradation via enzymatic processes for the production of valuable β-1,3-glucooligosaccharides (GOS) from curdlan has attracted considerable interest. CBM6E functions as a curdlan-specific β-1,3-endoglucanase, composed of a glycoside hydrolase family 128 (GH128) module and a carbohydrate-binding module (CBM) derived from family CBM6.

Results: Crystallographic analyses were conducted to comprehend the substrate specificity mechanism of CBM6E.

Silver nanoparticle-functionalized melamine-formaldehyde aerogel for online in-tube solid-phase microextraction of polycyclic aromatic hydrocarbons followed by HPLC-DAD analysis.

Based on the π-metal interaction between silver nanoparticles (AgNPs) and aromatic compounds, AgNPs were in-situ grown to melamine-formaldehyde (MF) aerogel for improving the extraction performance to polycyclic aromatic hydrocarbons (PAHs). The AgNPs/MF aerogel was regulated through varing the concentration of reactants, and characterized by scanning electron microscopy, X-ray photoelectron spectroscopy and X-ray powder diffraction. As a new extraction coating, the AgNPs/MF aerogel was coated to stainless-steel wires for in-tube solid-phase microextraction (IT-SPME).

Extraction improvement of ionic liquid-functionalized silica by in situ anion-exchange for online solid-phase extraction of estrogens in honey.

The accurate detection of analytes in honey is affected by the complex substrates, making it crucial to employ an effective sample preparation technique. In this work, an imidazolium ionic liquid was functionalized to the silica surface by a click reaction for solid-phase extraction (SPE) column, and in situ anion-exchange process was performed with different organic anions (dodecyl sulfonate, dodecyl benzene sulfonate, and naphthalene sulfonate). These SPE columns were evaluated through extracting the estrogens.

Core-shell silica@pyridyl conjugated microporous polymer as a stationary phase for high performance liquid chromatography.

Background: Because of the advantages of good selectivity, high sensitivity, and fast analysis, high performance liquid chromatography (HPLC) has become one of the modern analytical techniques in wide application range, such as biological analysis, environmental detection, pharmaceutical and food inspection, agriculture and other fields. The stationary phase greatly decides the chromatographic separation performance, so the development of novel stationary phase is most important for HPLC.

Results: Pyridyl conjugated microporous polymers (P-CMP) with one to four layers were modified on the surface of amino silica to obtain a novel core-shell material (SiO2@P-CMP) by the layer-by-layer assembly strategy and Chichibabin reaction.

Design, Synthesis, and Pharmacological Evaluation of Multisubstituted Pyrido[4,3-]pyrimidine Analogues Bearing Deuterated Methylene Linkers as Potent KRAS Inhibitors.

The breakthrough in drug development of KRAS inhibitors provides inspiration for targeting alternative KRAS mutations, especially the most prevalent KRAS variant. Based on the structural analysis of MRTX1133 in complex with KRAS, a comprehensive structure-activity study was conducted, which led to the discovery of several compounds (, , and ) that showed higher potency in suppressing the clonogenic growth of KRAS-dependent cancer cells. These new compounds markedly and selectively inhibited the binding of RBD peptide to GTP-bound KRAS with IC values between 0.

Biochar nanosphere-functionalized carbon fibers for in-tube solid-phase microextraction of polycyclic aromatic hydrocarbons in environmental water followed by liquid chromatography and diode array detection.

In order to improve the extraction ability of carbon fibers (CFs) for microextraction of polycyclic aromatic hydrocarbons (PAHs), biochar nanospheres derived from glucose were in-situ grown onto the surface of CFs via hydrothermal synthesis. The surface morphology and elemental composition of biochar nanospheres-CFs were investigated by scanning electron microscopy and X-ray photoelectron spectroscopy. Thereafter, the biochar nanosphere-CFs were pulled into the polyetheretherketone tube for solid-phase microextraction, and the tube was combined with high-performance liquid chromatography-diode array detector to online detect PAHs.

Comparative Transcriptome Profiling of CMS-D2 and CMS-D8 Systems Characterizes Fertility Restoration Genes Network in Upland Cotton.

Resolving the genetic basis of fertility restoration for cytoplasmic male sterility (CMS) can improve the efficiency of three-line hybrid breeding. However, the genetic determinants of male fertility restoration in cotton are still largely unknown. This study comprehensively compared the full-length transcripts of CMS-D2 and CMS-D8 systems to identify potential genes linked with fertility restorer genes or .

[Recent application advances of covalent organic frameworks for solid-phase extraction].

Covalent organic frameworks (COFs) are a type of crystalline porous polymers. It firstly prepared by thermodynamically controlled reversible polymerization to obtain chain units and connecting small organic molecular building units with a certain symmetry. These polymers are widely used in gas adsorption, catalysis, sensing, drug delivery, and many other fields.

MOF-Derived Mn O Nanocage with Oxygen Vacancies as Efficient Cathode Catalysts for Li-O Batteries.

Designing efficient and cost-effective electrocatalysts is the primary imperative for addressing the pivotal concerns confronting lithium-oxygen batteries (LOBs). The microstructure of the catalyst is one of the key factors that influence the catalytic performance. This study proceeds to the advantage of metal-organic frameworks (MOFs) derivatives by annealing manganese 1,2,3-triazolate (MET-2) at different temperatures to optimize Mn O crystals for special microstructures.

Discovery of a Potent and Oral Available Complex I OXPHOS Inhibitor That Abrogates Tumor Growth and Circumvents MEKi Resistance.

Targeting oxidative phosphorylation (OXPHOS) has emerged as a promising therapeutic strategy for cancer therapy. Here, we discovered a 1-1,2,3-triazole derivative as a highly potent and orally available OXPHOS inhibitor that effectively blocked the activity of mitochondrial complex I. specifically compromised the mitochondrial oxygen consumption of high-OXPHOS lung cancer cells but not that of low-OXPHOS lung cancer cells or normal cells in the low nanomolar range.

Feedback activation of EGFR/wild-type RAS signaling axis limits KRAS inhibitor efficacy in KRAS-mutated colorectal cancer.

Colorectal cancer (CRC), which shows a high degree of heterogeneity, is the third most deadly cancer worldwide. Mutational activation of KRAS occurs in approximately 10-12% of CRC cases, but the susceptibility of KRAS-mutated CRC to the recently discovered KRAS inhibitor MRTX1133 has not been fully defined. Here, we report that MRTX1133 treatment caused reversible growth arrest in KRAS-mutated CRC cells, accompanied by partial reactivation of RAS effector signaling.

Targeting metabolic vulnerability in mitochondria conquers MEK inhibitor resistance in -mutant lung cancer.

MEK is a canonical effector of mutant KRAS; however, MEK inhibitors fail to yield satisfactory clinical outcomes in -mutant cancers. Here, we identified mitochondrial oxidative phosphorylation (OXPHOS) induction as a profound metabolic alteration to confer -mutant non-small cell lung cancer (NSCLC) resistance to the clinical MEK inhibitor trametinib. Metabolic flux analysis demonstrated that pyruvate metabolism and fatty acid oxidation were markedly enhanced and coordinately powered the OXPHOS system in resistant cells after trametinib treatment, satisfying their energy demand and protecting them from apoptosis.