Automatic counting of retinal ganglion cells in the entire mouse retina based on improved YOLOv5.

Glaucoma is characterized by the progressive loss of retinal ganglion cells (RGCs), although the pathogenic mechanism remains largely unknown. To study the mechanism and assess RGC degradation, mouse models are often used to simulate human glaucoma and specific markers are used to label and quantify RGCs. However, manually counting RGCs is time-consuming and prone to distortion due to subjective bias.

Chemical Differentiation of Pseudostellariae Radix from Different Cultivated Fields and Germplasms by UPLC-Triple TOF-MS/MS Coupled with Multivariate Statistical Analysis.

To explore rapidly the potential chemical markers for differentiating Pseudostellariae Radix from different cultivated fields :and germplasms, a method is proposed based on ultra-performance liquid chromatographytriple time-of-flight mass/mass spectrometry (UPLC-Triple TOF-MS/MS) coupled with multivariate statistical analysis. Peak matching, peak alignment, and noise filtering were used in analyzing mass spectrometric data. Accurate m/z value analysis of MS data based on software of database search and MS/MS fragment analysis were applied to identify constituents.

[Correlation Between Index Components and Climatic Factors of Pseudostellariae Radix].

Objective: To analyze the correlation between index components and climatic factors of Pseudostellariae Radix.

Methods: Correlation analysis ( CA), partial least square (PLS) and PCA ordination were applied to analyze the correlation of index components in Pseudostellariae Radix and climatic factors, and to explore the main climatic factors affecting the accumulation of index components in Pseudostellariae Radix.

Results: The origin and harvest time had influence on index components.

[Simultaneous determination of thirteen components in Polygoni Multifori Caulis by UPLC-MS/MS].

A comprehensive analytical method based on UPLC-MS/MS was developed for the simultaneous determination of thirteen components including three stilbenes (stilbeneglucoside, polydatin, resveratrol), four anthraquinones (emodin, physcion, emodin-8-β-D-glucopyranoside, aloe-emodin), five flavonoids (epicatechin, rutin, hyperoside, astragalin,quercetin) and one phenolic acid (gallic acid) in Polygoni Multifori Caulis.The separation was carried out on a Waters BEH C18 column（2.1 mm×100 mm,1.

[iTRAQ-based quantitative proteomics of cultivated Pseudostellaria heterophylla and its wild type].

This work was designed to investigate proteins differentially expressed in cultivated Pseudostellaria heterophylla and its wild type using i TRAQ proteomics approach. The extracted proteins were digested using FASP method and identified by i TRAQ coupled with LC-MS/MS technology and then analyzed by Protein Pilot 5.0 search engine.

[Dynamic Changes of Nucleosides and Nucleobases in Different Harvest Periods of Polygoni Multiflori Radix by UPLC-QTRAP-MS/MS].

Objective: To analyze the dynamic changes of nucleosides and nucleobases in Polygoni Multiflori Radix harvested in different periods.

Methods: UPLC-QTRAP-MS/MS method was applied for the analysis of nine kinds of nucleosides and nucleobases in Polygoni Multiflori Radix.

Results: The content of uridine, adenine, guanosine and cytidine was higher in Polygoni Multiflori Radix harvested in different periods and assumed some difference.

Dynamic accumulation analysis on bioactive constituents of Polygonum multiflorum in different collection periods.

To study the dynamic change law of bioactive constituents from Polygonum multiflorum, and to explore the optimal harvest period of P. multiflorum. Determination of stilhene glucoside, anthraquinones and catechin from P.