Time course of global gene expression alterations in Candida albicans during infection of HeLa cells.

Candida albicans (C. albicans) is an opportunistic fungus that quickly adapts to various microniches. It causes candidiasis, a common fungal infection for which the pathogenic mechanism has not been elucidated yet.

[Clinical study of partial mole or choriocarcinoma with co-existing fetus].

Objective: To evaluate the rare entity of partial mole or choriocarcinoma with co-existing fetus.

Methods: A total of 7 cases of partial mole or choriocarcinoma with co-existing fetus were selected to collect clinical profiles and perform auxiliary examinations such as serum β-hCG and sonography. The data were analyzed retrospectively and all cases confirmed by surgery and histopathological diagnosis.

[Survey of gestational trophoblastic disease incidence among 3.6 million pregnancies in China].

Objective: To survey incidence of gestational trophoblastic disease (GTD) in China and to provide useful information for prevention and better treatment of the disease.

Methods: The survey was retrospectively carried out from 1991 to 2000 and included 143 hospitals in the following seven Chinese provinces: Zhejiang, Jiangsu, Fujian, Anhui, Jiangxi, Shanxi and Henan.

Results: Excluding incomplete data, data from 118 hospitals from seven provinces were finally analyzed.

[Changes in gene expression profiles of hydatidiform mole and choriocarcinoma as compared with trophoblast hyperplasia].

Objective: To study the relationship of changes in gene expression profiles of hydatidiform mole and choriocarcinoma with hyperplasia of trophoblasts.

Methods: The differentially expressed genes were analyzed in two pairs of tissues of hydatidiform mole versus normal villi, and in two pairs of normal primary culture trophoblasts versus JAR cell line of chariocarcinoma, using cDNA microarray containing 4096 genes. To confirm the results of cDNA microarray analysis, expressions of some up-regulated genes related to DNA synthesis in normal villi, hydatidiform mole, and 2 choriocarcinoma cell lines (JAR and JEG-3) were examined by immunohistochemistry, immunoblotting and RT-PCR.

[Inhibitory effect of tumor suppressor PTEN on cell growth of endometrial carcinoma].

Objective: To evaluate the inhibitory effect of tumor suppressor PTEN on cell growth of endometrial carcinoma.

Methods: The exogenous wild PTEN cDNA via an adenoviral vector (Ad-PTEN) was introduced into Ishikawa cells. The expression of PTEN protein was detected by Western blot.

[Preliminary study on gene expression profile of endometrial adenocarcinoma].

Objective: To determine candidate genes of endometrial adenocarcinoma.

Methods: To compare the gene expression profile in 2 endometrial adenocarcinoma tissues and 2 normal endometria by HGEC-40s GeneChip probe including 4096 genes array. Expression differences between normal and malignant tissue groups were measured by GenePixPro3.

[Study of DNA microarray chip of associated genes of hydatidiform mole].

Objective: To screen genes which may associate with the development and malignant transformation of hydatidiform mole.

Method: The differentially expressed genes were analyzed between the tissues of two cases of hydatidiform mole and the normal placental tissues of two cases of pregnancy which had almost the same pregnant ages as hydatidiform mole, using cDNA chips containing 4,096 genes.

Results: There were total 89 genes significantly differently expressed in all hydatidiform moles, counting for 2.