Health status and morphometrics of Galápagos magnificent frigatebirds () determined by hematology, biochemistry, blood gas, and physical examination.

The magnificent frigatebird ( MFB) is a widely distributed seabird. It has breeding areas in the tropical Atlantic Ocean and the Pacific Ocean (extending along Central America up to Baja California) (Schreiber & Burger, 2001). The (MFB-Gal) subspecies is native to the Galápagos Islands.

Assessing the chemical landscape of the Galápagos Marine Reserve.

The Galápagos Archipelago is at the forefront of the Anthropocene, facing intensifying pressures from its growing human footprint and accelerated global connectivity. Despite this, little is currently known of its chemical landscape. This review critically examines the drivers, sources, distribution and fate of oil, plastics, pesticides, persistent organic pollutants and heavy metals in the Galápagos Marine Reserve, identifying pollutant hotspots and evaluating rapid assessment methods and sentinel species that could aid regional monitoring.

Sea surface microplastics in the Galapagos: Grab samples reveal high concentrations of particles <200 μm in size.

Plastic pollution in the oceans is increasing, yet most global sea surface data is collected using plankton nets which limits our knowledge of the smaller and more bioaccessible size fraction of microplastics (<5 mm). We sampled the biodiverse coastal waters of the Galapagos Island of San Cristobal, comparing two different microplastic sampling methodologies; 1 l whole seawater grab samples filtered to 1.2 μm and sea surface plankton tows with a net mesh size of 200 μm.

Dried blood spot analysis for elements of nutritional concern as demonstrated in studies of Galápagos land iguanas (Conolophus species).

Background: Dried blood spot (DBS) technology is valuable in providing simple means of storing blood samples from wildlife with small blood volumes. Methods designed for heavy metal analysis on DBS become more useful if extended to elements of nutritional significance.

Purpose: (1) Development of procedures for measuring Mn, Fe, Co, Cu, Zn, Se and Mo in DBS; (2) use the designed methods in health assessments of Galápagos land iguanas (Conolophus species).

Endoscopy and rectal enema for fecal collection in wild sea turtles (Chelonia mydas, Eretmochelys imbricata) in a field setting.

Forty-seven free-ranging sea turtles (46- Chelonia mydas, 1- Eretmochelys imbricata) were examined via novel use of an endoscopy combined with a rectal enema to obtain large fecal sample volumes. The cloaca was insufflated using an endoscope, after which the bladder and rectum separated, allowing access to the colon. Environmental conditions and location influenced the performance of the procedure initially, but after several attempts the procedure was successfully initiated.

Detection and quantification of microplastic pollution in the endangered Galapagos sea lion.

Marine debris pollution poses a significant global threat to biodiversity, with plastics being the primary debris type found in oceans due to their low-cost production and high demand worldwide. Microplastics (MPs, <5 mm in size) are highly bioavailable to a wide range of marine taxa, including marine mammals, through direct and indirect ingestion routes (i.e.

Health status of the red-billed tropicbird () determined by hematology, biochemistry, blood gases, and physical examination.

The red-billed tropicbird, , is a species of seabird native to the Galápagos archipelago, and widely distributed across the neotropics. General health, blood chemistry, and haematology parameters have not been published for this species. Blood analyses were performed on samples drawn from 51 clinically healthy red-billed tropicbirds captured from their burrows at Islote Pitt on San Cristóbal Island in July, 2016 (21) and Daphne Major Island in June, 2017 (30).

Microplastic distribution and composition on two Galápagos island beaches, Ecuador: Verifying the use of citizen science derived data in long-term monitoring.

Monitoring beach plastic contamination across space and time is necessary for understanding its sources and ecological effects, and for guiding mitigation. This is logistically and financially challenging, especially for microplastics. Citizen science represents an option for sampling accessible sites to support long term monitoring, but challenges persist around data validation.

Multiple anthropogenic stressors in the Galápagos Islands' complex social-ecological system: Interactions of marine pollution, fishing pressure, and climate change with management recommendations.

For decades, multiple anthropogenic stressors have threatened the Galápagos Islands. Widespread marine pollution such as oil spills, persistent organic pollutants, metals, and ocean plastic pollution has been linked to concerning changes in the ecophysiology and health of Galápagos species. Simultaneously, illegal, unreported, and unregulated fishing are reshaping the composition and structure of endemic and native Galápagos pelagic communities.

Evaluating the Possibility of Transfusion Medicine, Through Crossmatching in Juvenile Galapagos Sea Lions ().

The Galapagos sea lion () is an endemic pinniped to the Galapagos archipelago, and like most wild mammals, is at risk for anemia due to trauma, infectious disease, and poor nutrition. This study evaluated the health status of 26 juvenile Galapagos sea lions on the island of San Cristobal prior to evaluating 100 crossmatch combinations. On evaluation, all but one sea lion had no major systemic abnormalities.

Baseline haematology, biochemistry, blood gas values and health status of the Galapagos swallow-tailed gull ().

The swallow-tailed gull, , is a seabird endemic to the Galápagos archipelago. In general health, blood chemistry and haematology, parameters have not been published for this species. Blood analyses were run on samples drawn from 58 clinically healthy swallow-tailed gulls captured at Islote Pitt on San Cristóbal Island in July 2016 (28) and South Plaza Island in June 2017 (30).

International fisheries threaten globally endangered sharks in the Eastern Tropical Pacific Ocean: the case of the Fu Yuan Yu Leng 999 reefer vessel seized within the Galápagos Marine Reserve.

Shark fishing, driven by the fin trade, is the primary cause of global shark population declines. Here, we present a case study that exemplifies how industrial fisheries are likely depleting shark populations in the Eastern Tropical Pacific Ocean. In August 2017, the vessel Fu Yuan Yu Leng 999, of Chinese flag, was detained while crossing through the Galápagos Marine Reserve without authorization.

Whole blood fatty acid concentrations in the San Cristóbal Galápagos tortoise .

To continue releasing San Cristóbal Galápagos tortoises housed in managed-care facilities at the Giant Tortoise Breeding Center of Galápagos National Park (Galapaguera de Cerro Colorado) to the Otoy Ecological Farm, health assessments and physical examinations were conducted. As a part of these wellness examinations, blood was drawn from 11 tortoises to analyze fatty acid concentrations. Fatty acid levels can provide insight into the nutritional profiles, immune status, and reproductive health of vertebrates.

Green, yellow or black? Genetic differentiation and adaptation signatures in a highly migratory marine turtle.

Marine species may exhibit genetic structure accompanied by phenotypic differentiation related to adaptation despite their high mobility. Two shape-based morphotypes have been identified for the green turtle () in the Pacific Ocean: the south-central/western or yellow turtle and north-central/eastern or black turtle. The genetic differentiation between these morphotypes and the adaptation of the black turtle to environmentally contrasting conditions of the eastern Pacific region has remained a mystery for decades.

HEALTH STATUS OF NAZCA BOOBIES () ON DAPHNE MAJOR ISLAND IN THE GALÁPAGOS DETERMINED BY HEMATOLOGY, BIOCHEMISTRY, AND PHYSICAL EXAMINATION.

Island species are particularly vulnerable to environmental disturbances and introduced pathogens. Conducting health assessments of wild populations in the Galápagos improves the ability of wildlife managers and veterinarians to detect deteriorations in health status. Seabirds in particular are useful species to monitor due to their colonial breeding and wide migration range.

Plastic contamination of a Galapagos Island (Ecuador) and the relative risks to native marine species.

Ecuador's Galapagos Islands and their unique biodiversity are a global conservation priority. We explored the presence, composition and environmental drivers of plastic contamination across the marine ecosystem at an island scale, investigated uptake in marine invertebrates and designed a systematic priority scoring analysis to identify the most vulnerable vertebrate species. Beach contamination varied by site (macroplastic 0-0.

Targeted therapy for pediatric low-grade glioma.

Purpose: Pediatric low-grade gliomas are the most frequent brain tumors in children. The standard approach for symptomatic unresectable tumors is chemotherapy. Recently, key molecular alterations/pathways have been identified and targeted drugs developed and tested in clinical trials.

HEALTH STATUS AND BASELINE HEMATOLOGY, BIOCHEMISTRY, AND BLOOD GAS VALUES OF GALAPAGOS SHEARWATERS ().

The Galápagos shearwater, , is a seabird endemic to the Galápagos archipelago. Hematology, blood chemistry, and general health parameters have not been published for this species. Analyses were run on blood samples drawn from 20 clinically healthy Galápagos shearwaters captured by hand at their nests at Islote Pitt on San Cristóbal Island in July 2016.

Health assessment of Conolophus subcristatus, Conolophus pallidus, and C. subcristatus X Amblyrhynchus cristatus hybrid (Galápagos land iguanas).

The land iguanas, Conolophus pallidus and Conolophus subcristatu are large and charismatic lizards endemic to the Galápagos archipelago, but little information exists on their normal health parameters. The former is restricted to Santa Fe island, while C. subcristatus inhabits the islands of the central and western region of the archipelago.

Methods of body temperature assessment in , (Galápagos land iguanas), and X hybrid.

Since cardiovascular, respiratory, and metabolic systems of reptiles are affected by temperature, accurate measurements are of great importance in both captive husbandry and research. Ectothermic animals generally have core body temperatures close to ambient temperature but can differ from the immediate environment if they are using sunlight to thermoregulate. Many zoological facilities and exotic pet caregivers have begun using infrared temperature guns to assess ambient temperatures of reptile enclosures but there are currently few studies assessing the efficacy of these devices for measuring the body temperatures of reptiles.

Haematology and biochemistry of the San Cristóbal Lava Lizard ().

The San Cristóbal lava lizard, , is one of nine species of lava lizards endemic to the Galápagos Islands of Ecuador. No information presently exists about baseline health parameters for any of these species. We analysed blood samples drawn from 47 lizards (25 males and 22 females) captured at two locations on San Cristóbal Island.

Health Status of Great Frigatebirds () Determined by Haematology, Biochemistry, Blood Gases, and Physical Examination.

The great frigatebird, , is a widely distributed seabird native to the Galápagos archipelago. Haematology and blood chemistry parameters have been published for this species but not from the San Cristóbal and North Seymour great frigatebird breeding colonies. Analyses were run on blood samples drawn from 25 great frigatebirds captured by hand at their nests at Punta Pitt on San Cristóbal Island and 30 birds on North Seymour Island, Galápagos Islands.

Biochemistry and hematology parameters of the San Cristóbal Galápagos tortoise ().

As part of a planned introduction of captive Galapagos tortoises () to the San Cristóbal highland farms, our veterinary team performed thorough physical examinations and health assessments of 32 tortoises. Blood samples were collected for packed cell volume (PCV), total solids (TS), white blood cell count (WBC) differential, estimated WBC and a biochemistry panel including lactate. In some cases not all of the values were obtainable but most of the tortoises have full complements of results.

HEALTH STATUS OF RED-FOOTED BOOBIES ( SULA SULA) DETERMINED BY HEMATOLOGY, BIOCHEMISTRY, BLOOD GASES, AND PHYSICAL EXAMINATION.

The red-footed booby ( Sula sula) is a widely distributed sulid native to the Galápagos archipelago. Hematology and blood chemistry parameters have been published for this species, but not from the San Cristóbal rookery. Analyses were run on blood samples drawn from 31 manually restrained red-footed boobies that were captured by hand from their nests at Punta Pitt on San Cristóbal Island.