Publications by authors named "Juan Perez-Roldan"
Chromatin-associated RNAs (cRNAs) are a poorly characterized fraction of cellular RNAs that co-purify with chromatin. Their full complexity and the mechanisms regulating their packaging and chromatin association remain poorly understood. Here, we address these questions in Drosophila.
View Article and Find Full Text PDFArticle Synopsis
- Chromosome pairing is crucial for genome organization, but the mechanisms in somatic cells, particularly in Drosophila, are not well understood, despite their pronounced pairing features.
- The study identifies two factors that influence the localization of CAP-H2, an anti-pairing protein, particularly focusing on Z4's role in its function at architectural protein binding sites (APBSs).
- Additionally, they discover that osmotic stress can cause rapid unpairing of chromosomes, emphasizing the relationship between chromosome pairing and different types of 3D interactions within the genome.
In the nucleus, chromatin is intricately structured into multiple layers of 3D organization important for genome activity. How distinct layers influence each other is not well understood. In particular, the contribution of chromosome pairing to 3D chromatin organization has been largely neglected.
View Article and Find Full Text PDFTools for actively targeted DNA demethylation are required to increase our knowledge about regulation and specific functions of this important epigenetic modification. DNA demethylation in mammals involves TET-mediated oxidation of 5-methylcytosine (5-meC), which may promote its replication-dependent dilution and/or active removal through base excision repair (BER). However, it is still unclear whether oxidized derivatives of 5-meC are simply DNA demethylation intermediates or rather epigenetic marks on their own.
View Article and Find Full Text PDF