Transcriptome and metabolome analysis reveal the mechanisms of iron absorption differences in apple rootstocks under alkaline condition.

Iron (Fe) is an essential micronutrient for plant growth and development. Fe deficiency leads to growth restriction, developmental disorders, chlorosis, and yield loss of fruit trees. This study investigated the molecular and biochemical mechanisms underlying the differences in Fe absorption among various apple rootstocks under alkaline conditions.

Identification of the grape MAPKKK gene family and functional analysis of the VaMAPKKK15 gene under low temperature stress.

Mitogen-activated protein kinase kinase kinases (MAPKKK, MAP3K, MKKK or MEKK) regulate plant growth and development and are involved in regulatory pathways for plant resistance to a wide range of biotic and abiotic stresses. However, the phylogeny of the MAPKKK gene family and the biological function of VaMAPKKK15 under low-temperature stress in Vitis vinifera (V. vinifera) and Vitis amurensis (V.

Preparation of a tetraethylenepentamine grafted magnetic chitosan bead for adsorption of Re(vii) from aqueous solutions.

A cross-linked magnetic chitosan bead (MCB) and tetraethylenepentamine grafted magnetic chitosan bead (TMCB) were synthesized using the water/oil (W/O) emulsion cross-linking method and characterized using scanning electron microscopy (SEM), vibrating-sample magnetometry (VSM) and Fourier transform infrared spectroscopy (FTIR). The saturation magnetization and remanent magnetization of TMCB were 7.09 emu g and 0.

NcBRI1 positively regulate vascular development and promote biomass production in Neolamarckia cadamba.

Brassinosteroids (BRs) are essential phytohormones that play a crucial role in plant growth and development. However, our understanding of BR receptors and their functions in tree species is currently limited. In this study, we looked for potential BR receptor genes in the burflower-tree (Neolamarckia cadamba) genome.

The role of gibberellin synthase gene VvGA2ox7 acts as a positive regulator to salt stress in Arabidopsis thaliana.

Background: Soil salinity is an important environmental component affecting plant growth and yield, but high-salinity soils are a major constraint to the development of the grape industry. Previous studies have provided lines of evidence that gibberellins (GAs) play a significant regulatory role in plant responses to salt stress. However, it remains unclear whether GA2ox, a key enzyme that maintains the balance of bioactive gibberellins and intermediates in plants, is involved in the mechanism of salt stress tolerance in grapes.

Integration of mRNA-miRNA Reveals the Possible Role of in Increasing Branches Through Bud-Notching in Pear ( Rehd.).

Bud-notching in pear varieties with weak-branches enhances branch development, hormone distribution, and germination, promoting healthier growth and improving early yield. To examine the regulatory mechanisms of endogenous hormones on lateral bud germination in spp. (cv.

Machine learning-assisted source tracing in domestic-industrial wastewater: A fluorescence information-based approach.

An emergency water pollution incident poses a significant risk to the proper functioning of wastewater treatment plants, particularly in domestic-industrial integrated facilities. Source tracing is recognized as an effective method to mitigate ongoing impacts. Machine learning-assisted traceability is emerging as a more efficient and faster method compared to traditional methods.

Expression analysis of the apple HSP70 gene family in abiotic stress and phytohormones and expression validation of candidate MdHSP70 genes.

Heat shock protein 70 (HSP70) is one kind of molecular chaperones which are widely found in organisms, and its members are highly conserved among each other, with important roles in plant growth and development. In this study, 56 HSP70 genes were identified from the apple genome database. Analysis of gene duplication events showed that tandem and segmental duplication events play an important role in promoting the amplification of the MdHSP70 gene family.

The gapless genome assembly and multi-omics analyses unveil a pivotal regulatory mechanism of oil biosynthesis in the olive tree.

Olive is a valuable oil-bearing tree with fruits containing high levels of fatty acids. Oil production is a multifaceted process involving intricate interactions between fatty acid biosynthesis and other metabolic pathways that are affected by genetics and the developmental stages of the fruit. However, a comprehensive understanding of the underlying regulatory mechanisms is still lacking.

Interaction of the Transcription Factors BES1/BZR1 in Plant Growth and Stress Response.

Bri1-EMS Suppressor 1 (BES1) and Brassinazole Resistant 1 (BZR1) are two key transcription factors in the brassinosteroid (BR) signaling pathway, serving as crucial integrators that connect various signaling pathways in plants. Extensive genetic and biochemical studies have revealed that BES1 and BZR1, along with other protein factors, form a complex interaction network that governs plant growth, development, and stress tolerance. Among the interactome of BES1 and BZR1, several proteins involved in posttranslational modifications play a key role in modifying the stability, abundance, and transcriptional activity of BES1 and BZR1.

Post-translational Regulation of BRI1-EMS Suppressor 1 and Brassinazole-Resistant 1.

Brassinosteroid-insensitive 1 (BRI1)-EMS suppressor 1 (BES1) and Brassinazole-resistant 1 (BZR1) are two highly similar master transcription factors of the brassinosteroid (BR) signaling pathway that regulates a variety of plant growth and development processes as well as stress responses. Previous genetic and biochemical analyses have established a complex regulatory network to control the two transcription factors. This network includes coordination with other transcription factors and interactors, multiple post-translational modifications (PTMs) and differential subcellular localizations.

The asymmetric expression of plasma membrane H-ATPase family genes in response to pulvinus-driven leaf phototropism movement in Vitis vinifera.

Phototropism movement is crucial for plants to adapt to various environmental changes. Plant P-type H-ATPase (HA) plays diverse roles in signal transduction during cell expansion, regulation of cellular osmotic potential and stomatal opening, and circadian movement. Despite numerous studies on the genome-wide analysis of Vitis vinifera, no research has been done on the P-type H-ATPase family genes, especially concerning pulvinus-driven leaf movement.

Molecular Evolution of SNAREs in and Expression Analysis under Phytohormones and Abiotic Stress.

SNARE proteins (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) play a key role in mediating a variety of plant biological processes. Currently, the function of the SNARE gene family in phytohormonal and abiotic stress treatments in grapevine is currently unknown, making it worthwhile to characterize and analyze the function and expression of this family in grapevine. In the present study, 52 genes were identified and predominantly distributed on 18 chromosomes.

Overexpression of VaBAM3 from Vitis amurensis enhances seedling cold tolerance by promoting soluble sugar accumulation and reactive oxygen scavenging.

The VaBAM3 cloned from Vitis amurensis can enhance the cold tolerance of overexpressed plants, but VaBAM3 knock out by CRISPR/Cas9 system weakened grape callus cold tolerance. In grape production, extreme cold conditions can seriously threaten plant survival and fruit quality. Regulation of starch content by β-amylase (BAM, EC: 3.

MdbZIP44-MdCPRF2-like- regulate starch and sugar metabolism in apple under nitrogen supply.

Nitrogen (N) is regarded as an essential macronutrient and is tightly associated with carbon (C) metabolism in plants. The transcriptome data obtained from this study showed that the expression level of the apple basic leucine zipper (bZIP) transcription factor (TF) was up-regulated in 'Oregon Spur Delicious' ( Borkh.) apple fruits under nitrogen supply.

Positively Regulates Cold Tolerance in and Grape.

Cold stress adversely impacts grape growth, development, and yield. Therefore, improving the cold tolerance of grape is an urgent task of grape breeding. The Jasmonic acid (JA) pathway responsive gene plays a key role in plant response to cold stress.

Optimized design of quaternary amino-functionalized chitosan fibers for ultra-high diclofenac adsorption from wastewater.

The extraction of non-steroidal anti-inflammatory drugs (NSAIDs) from water bodies is imperative due to the potential harm to humans and the ecosystem caused by NSAID-contaminated water. Quaternary amino-functionalized epichlorohydrin cross-linked chitosan fibers (QECFs), an economical and eco-friendly adsorbent, were successfully prepared using a simple and gentle method for efficient diclofenac (DCF) adsorption. Additionally, the optimized factors for the preparation of QECFs included epichlorohydrin concentration, pH, temperature, and (3-chloro-2-hydroxypropyl) trimethylammonium chloride (CHTAC) concentration.

Regulatory mechanism of GA application on grape (Vitis vinifera L.) berry size.

Gibberellin A (GA) is often used as a principal growth regulator to increase plant size. Here, we applied Tween-20 (2%)-formulated GA (T1:40 mg/L; T2:70 mg/L) by dipping the clusters at the initial expansion phase of 'Red Globe' grape (Vitis vinifera L.) in 2018 and 2019.

ZNF460-mediated circRPPH1 promotes TNBC progression through ITGA5-induced FAK/PI3K/AKT activation in a ceRNA manner.

Background: Circular RNAs are highly stable regulatory RNAs that have been increasingly associated with tumorigenesis and progression. However, the role of many circRNAs in triple-negative breast cancer (TNBC) and the related mechanisms have not been elucidated.

Methods: In this study, we screened circRNAs with significant expression differences in the RNA sequencing datasets of TNBC and normal breast tissues and then detected the expression level of circRPPH1 by qRT‒PCR.