Publications by authors named "Juan Jordan-Chaves"
Development and Validation of a Highly Sensitive Multiplex Immunoassay for SARS-CoV-2 Humoral Response Monitorization: A Study of the Antibody Response in COVID-19 Patients with Different Clinical Profiles during the First and Second Waves in Cadiz, Spain.
Lucia Olvera-Collantes Noelia Moares Ricardo Fernandez-Cisnal Juan P Muñoz-Miranda Pablo Gonzalez-Garcia Juan de Dios Jordan-Chaves
Microorganisms
December 2023
Article Synopsis
- The COVID-19 pandemic continues to challenge healthcare systems with emerging waves, necessitating new preventive tools and therapies.
- A novel immunoassay using Bio-Plex technology has been developed to simultaneously detect multiple IgG antibodies against different SARS-CoV-2 antigens, showcasing high sensitivity and specificity.
- This multiplex immunoassay serves as a robust tool for monitoring humoral immunity and can aid in epidemiological surveillance, particularly in analyzing antibody responses among unvaccinated COVID-19 patients in Cadiz, Spain.
Background: The aim was clinical evaluation of immune response against SARS-CoV-2, analyzing serum levels of IgG antibodies against the SARS-CoV-2 protein S in infected and vaccinated patients, as well as in subjects with and without frequent comorbidities (arterial hypertension, diabetes mellitus, heart disease, and chronic respiratory disease).
Methods: Patients infected by SARS-CoV-2 confirmed by RT-PCR and subjects vaccinated with vaccines based on the mRNA encoding the SARS-CoV-2 protein S were studied. SARS-CoV-2 anti-S IgG serum levels were quantified by chemiluminescent microparticle immunoassay.