Detection of Fumonisins in Fusarium Cultures, Corn, and Corn Products by Polyclonal Antibody-Based ELISA: Relation to Fumonisin B Detection by Liquid Chromatography.

A specific sheep polyclonal antiserum was applied to the competitive direct enzyme-linked immunosorbent assay (CD-ELISA) of fumonisins in Fusarium cultures, fresh corn, animal feed, and human foods, and the results were related to fumonisin B (FB) levels determined by liquid chromatography (LC). The limit of detection of FB for the CD-ELISA was 0.1 ng/ml.

Comparative Assessment of Fumonisin in Grain-Based Foods by ELISA, GC-MS, and HPLC.

Seventy-one (71) food samples were analyzed for the mycotoxin fumonisin by a monoclonal antibody based competitive enzyme-linked immunosorbent assay (ELISA). Fumonisins were detected primarily in corn-based products with 7/12, 2/2 and 1/3 and 1/7 yellow cornmeal, blue cornmeal, corn muffin mix, and mixed grain cereal samples yielding positive results, respectively. When the positive samples and randomly selected negative samples were assessed by other methods, correlations (r values) between ELISA and gas chromatography-mass spectrometry (GC-MS), ELISA and high-pressure liquid chromatography (HPLC) and GC-MS and HPLC were 0.