Detection of Bovine Milk in Ovine Milk by a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA).

A sandwich ELISA (enzyme-linked immunosorbent assay) was developed for the detection of defined amounts of bovine milk (1-30%) in ovine milk. Polyclonal antibodies were raised in rabbits against bovine whey proteins (BWP). Resultant antibodies were affinity purified by immunoadsorption of the crude antiserum onto columns containing immobilized ovine, caprine, and BWP, followed by elution of the bovine milk specific antibodies (anti-BWP) from the column containing the bovine proteins.

Detection of Zearalenone By Tandem Immunoaffinity-Enzyme-Linked Immunosorbent Assay and Its Application to Milk.

A hybridoma-based method utilizing tandem affinity chromatography and enzyme-linked immunosorbent assay (ELISA) was devised to detect zearalenone. A zearalenone specific monoclonal antibody was attached to Sepharose for initial sample clean-up. Zearalenone was eluted with methanol and then quantified by competitive direct ELISA.

Sandwich ELISA for Detection of Pig Meat in Raw Beef Using Antisera to Muscle Soluble Proteins.

A double-antibody sandwich ELISA (enzyme-linked immunosorbent assay) has been successfully developed for the detection of defined amounts of pig meat (1-50%) in raw beef. Antibodies against pig sarcoplasmic extracts were produced in rabbits. Pig-specific antibodies were affinity purified by removing antibodies which crossreacted with horse, chicken or beef extracts followed by immunoadsorption and elution from a pig-extract column.