Publications by authors named "Juan H Doria"
Article Synopsis
- The study focused on the Escherichia coli O157:H7 strain 02-0304 to identify genes that may facilitate the breakdown of sinigrin, a compound found in Oriental mustard, and linked this process to the enzyme myrosinase.
- In-depth analyses indicated that certain E. coli genes (bglA, ascB, and chbF) showed significant similarity to plant myrosinase, and their presence was confirmed through PCR testing.
- Gene disruption experiments revealed that bglA and ascB are crucial for the breakdown of sinigrin in this particular strain of E. coli, highlighting their importance in hydrolyzing the compound into its bactericidal form.
Allyl isothiocyanate (AITC) is an essential oil with antimicrobial activity against Escherichia coli O157:H7. The ability of E. coli O157:H7 to withstand inhibitory AITC concentrations and the role of the two-component BaeSR system as a defense mechanism against AITC was studied.
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