Integrated Microfluidic-Electromagnetic System to Probe Single-Cell Magnetotaxis in Microconfinement.

Magnetotactic bacteria have great potential for use in biomedical and environmental applications due to the ability to direct their navigation with a magnetic field. Applying and accurately controlling a magnetic field within a microscopic region during bacterial magnetotaxis studies at the single-cell level is challenging due to bulky microscope components and the inherent curvilinear field lines produced by commonly used bar magnets. In this paper, a system that integrates microfluidics and electromagnetic coils is presented for generating a linear magnetic field within a microenvironment compatible with microfluidics, enabling magnetotaxis analysis of groups or single microorganisms on-chip.

Hybrid plasmonic metasurface as enhanced Raman hot-spots for pesticide detection at ultralow concentrations.

A surface-enhanced Raman scattering (SERS) active metasurface composed of metallic nanohole arrays and metallic nanoparticles is developed. The metasurface can operate in aqueous environments, achieves an enhancement factor of 1.83 × 10 for Rhodamine 6G, and enables the detection of malachite green at a concentation of 0.

FDTD Analysis of Hotspot-Enabling Hybrid Nanohole-Nanoparticle Structures for SERS Detection.

Metallic nanoparticles (MNPs) and metallic nanostructures are both commonly used, independently, as SERS substrates due to their enhanced plasmonic activity. In this work, we introduce and investigate a hybrid nanostructure with strong SERS activity that benefits from the collective plasmonic response of the combination of MNPs and flow-through nanohole arrays (NHAs). The electric field distribution and electromagnetic enhancement factor of hybrid structures composed of silver NPs on both silver and gold NHAs are investigated via finite-difference time-domain (FDTD) analyses.

Cicada Wing Inspired Template-Stripped SERS Active 3D Metallic Nanostructures for the Detection of Toxic Substances.

This article introduces a bioinspired, cicada wing-like surface-enhanced Raman scattering (SERS) substrate based on template-stripped crossed surface relief grating (TS-CSRG). The substrate is polarization-independent, has tunable nanofeatures and can be fabricated in a cleanroom-free environment via holographic exposure followed by template-stripping using a UV-curable resin. The bioinspired nanostructures in the substrate are strategically designed to minimize the reflection of light for wavelengths shorter than their periodicity, promoting enhanced plasmonic regions for the Raman excitation wavelength at 632.

Structural Stability of Optofluidic Nanostructures in Flow-Through Operation.

Optofluidic sensors based on periodic arrays of subwavelength apertures that support surface plasmon resonance can be employed as both optical sensors and nanofluidic structures. In flow-through operation, the nanoapertures experience pressure differences across the substrate in which they are fabricated, which imposes the risk for structural failure. This work presents an investigation of the deflection and structural stability of nanohole array-based optofluidic sensors operating in flow-through mode.

Rapid label-free detection of intact pathogenic bacteria in situ via surface plasmon resonance imaging enabled by crossed surface relief gratings.

The unique plasmonic energy exchange occurring within metallic crossed surface relief gratings (CSRGs) has recently motivated their use as biosensors. However, CSRG-based biosensing has been limited to spectroscopic techniques, failing to harness their potential for integration with ubiquitous portable electronics. Here, we introduce biosensing via surface plasmon resonance imaging (SPRi) enabled by CSRGs.

Selective Uropathogenic Detection Using Crossed Surface-Relief Gratings.

Urinary tract infections (UTIs) are one of the major burdens on public healthcare worldwide. One of the primary causes of UTIs is the invasion of the urinary tract by uropathogenic (UPEC). Improper treatment of bacterial infections like UTIs with broad-spectrum antibiotics has contributed to the rise of antimicrobial resistance, necessitating the development of an inexpensive, rapid and accurate detection of UPEC.

Cost-effective flow-through nanohole array-based biosensing platform for the label-free detection of uropathogenic E. coli in real time.

Rapid, inexpensive and sensitive detection of uropathogenic Escherichia coli (UPEC), a common cause of ascending urinary tract infections (UTIs) including cystitis and pyelonephritis, is critical given the increasing number of cases and its recurrence worldwide. In this paper, we present a label-free nanoplasmonic sensing platform, built with off-the-shelf optical and electronic components, which can detect intact UPEC at concentrations lower than the physiological limit for UTI diagnosis, in real time. The sensing platform consists of a red LED light source, lens assembly, CMOS detector, Raspberry Pi interface in conjugation with a metallic flow-through nanohole array-based sensor.