A continuous flow bioreactor system for high-throughput hyperpolarized metabolic flux analysis.

Hyperpolarized carbon-13 labeled compounds are increasingly being used in medical MR imaging (MRI) and MR imaging (MRI) and spectroscopy (MRS) research, due to its ability to monitor tissue and cell metabolism in real-time. Although radiological biomarkers are increasingly being considered as clinical indicators, biopsies are still considered the gold standard for a large variety of indications. Bioreactor systems can play an important role in biopsy examinations because of their ability to provide a physiochemical environment that is conducive for therapeutic response monitoring ex vivo.

Advances in the discovery of heterocyclic-based drugs against Alzheimer's disease.

Introduction: Alzheimer's disease is a multifactorial neurodegenerative disorder characterized by beta-amyloid accumulation and tau protein hyperphosphorylation. The disease involves interconnected mechanisms, which can be clustered into two target-packs based on the affected proteins. Pack-1 focuses on beta-amyloid accumulation, oxidative stress, and metal homeostasis dysfunction, and Pack-2 involves tau protein, calcium homeostasis, and neuroinflammation.

Curcumin-Piperlongumine Hybrids with a Multitarget Profile Elicit Neuroprotection in In Vitro Models of Oxidative Stress and Hyperphosphorylation.

Curcumin shows a broad spectrum of activities of relevance in the treatment of Alzheimer's disease (AD); however, it is poorly absorbed and is also chemically and metabolically unstable, leading to a very low oral bioavailability. A small library of hybrid compounds designed as curcumin analogues and incorporating the key structural fragment of piperlongumine, a natural neuroinflammation inhibitor, were synthesized by a two-step route that combines a three-component reaction between primary amines, β-ketoesters and α-haloesters and a base-promoted acylation with cinnamoyl chlorides. These compounds were predicted to have good oral absorption and CNS permeation, had good scavenging properties in the in vitro DPPH experiment and in a cellular assay based on the oxidation of dichlorofluorescin to a fluorescent species.

Sensitive optomechanical transduction of electric and magnetic signals to the optical domain.

We report a radio-frequency-to-optical converter based on an electro-optomechanical transduction scheme where the electrical, optical, and mechanical interface was integrated on a chip and operated with a fiber-coupled optical setup. The device was designed for field tests in a magnetic resonance scanner where its small form-factor and simple operation is paramount. For the appurtenant magnetic resonance detection circuit at 32 MHz, we demonstrate transduction with an intrinsic magnetic field sensitivity of 8 fT/Hz, noise figure 2.

Use of sonic tomography to detect and quantify wood decay in living trees.

Premise Of The Study: Field methodology and image analysis protocols using acoustic tomography were developed and evaluated as a tool to estimate the amount of internal decay and damage of living trees, with special attention to tropical rainforest trees with irregular trunk shapes.

Methods And Results: Living trunks of a diversity of tree species in tropical rainforests in the Republic of Panama were scanned using an Argus Electronic PiCUS 3 Sonic Tomograph and evaluated for the amount and patterns of internal decay. A protocol using ImageJ analysis software was used to quantify the proportions of intact and compromised wood.

Assessment of English language development: a validity study of a district initiative.

The San Francisco Unified School District (SFUSD) uses the Language and Literacy Assessment Rubric (LALAR) as the secondary measurement required by the No Child Left Behind (NCLB) Act to measure English proficiency of English language learners (ELLs). In this analysis, the Rasch model is used to identify whether the LALAR is a valid measurement instrument and scale to measure the "English proficiency" of ELLs. This analysis investigates the relationship between student ability () and the probability that the student will respond correctly to an item on the LALAR.