The Killer Toxin: From Origin to Biomedical Research.

The systems of are defined by the co-infection of two viral agents, an M virus and a helper virus. Each toxin is determined by the type of M virus (ScV-M1, ScV-M2, ScV-M28, and ScV-Mlus), which encodes a specific toxin (K1, K2, K28, and Klus). Since their discovery, interest in their potential use as antimicrobial agents has driven research into the mechanisms of action of these toxins on susceptible cells.

Biocontrol Activity of CH05 and CH13 Isolated from L. Seeds against Fungal Strains.

In this study, seed-surface-associated bacteria from fresh fruits of spp. were analyzed to explore potential isolates for biocontrol of phytopathogenic fungal strains. A total of 76 bacterial isolates were obtained from three different species of chili pepper ( L.

Phaseolotoxin: Environmental Conditions and Regulatory Mechanisms Involved in Its Synthesis.

Phaseolotoxin is an antimetabolite toxin produced by diverse pathovars of Pseudomonas syringae which affects various plants, causing diseases of economic importance. Phaseolotoxin contributes to the systemic dissemination of the pathogen in the plant, therefore it is recognized as a major virulence factor. Genetic traits such as the Pht cluster, appear defining to the toxigenic strains phaseolotoxin producers.

Role of Volatile Organic Compounds Produced by Cp1 during Competitive Colonization Interaction against SM2.

The competitive colonization of bacteria on similar ecological niches has a significant impact during their establishment. The synthesis speeds of different chemical classes of molecules during early competitive colonization can reduce the number of competitors through metabolic effects. In this work, we demonstrate for the first time that Cp1 previously isolated from the seeds of R.

Electrostatic Fermentation: Molecular Response Insights for Tailored Beer Production.

Electrostatic fermentation avoids the cellular redox imbalance of traditional fermentation, but knowledge gaps exist. This study explores the impact of electrostatic fermentation on the growth, volatile profile, and genetic response of Saflager S-23. The applied voltage (15 and 30 V) in the electrostatic fermentation system increased the growth and substrate utilization of while decreasing ethanol production.

A Study of Resistome in Mexican Chili Powder as a Public Health Risk Factor.

Chili powder is an important condiment around the world. However, according to various reports, the presence of pathogenic microorganisms could present a public health risk factor during its consumption. Therefore, microbiological quality assessment is required to understand key microbial functional traits, such as antibiotic resistance genes (ARGs).

Metagenome-assembled bacterial genomes recovered from the datasets of (Smith) (Lepidoptera: Noctuidae).

(Smith) (Lepidoptera: Noctuidae), also known as the fall armyworm, is an economically important and widespread polyphagous pest. Microorganisms associated to this insect during life cycle play important ecological roles. We report 3 metagenome-assembled bacterial genomes reconstructed from a metagenome dataset obtained from larvae F3 3rd-instar reared using artificial diet under laboratory conditions.

Evaluation of the Insecticidal Potential of Acetonic and Methanolic Extracts against and Their Ecotoxicological Effect on .

For the management of , botanical extracts have been used to reduce the environmental impacts of synthetic chemical pesticides. In the present investigation, the insecticidal activity of the acetonic and methanolic extracts of (Asteraceae) and of the main compound 7-hydroxy-3,4-dihydrocadalene on this pest as well as its ecotoxicological effect on were evaluated. A greater insecticidal response was obtained from the acetonic extracts than from the methanolic extracts, with LC values of 730.

Volatile Organic Compounds Produced by Cp1 Isolated from the Seeds of R & P Possess Antifungal Activity.

The Cp1 strain was isolated from seeds of R. & P. cultivated in Michoacan, Mexico.

Ch1 Isolated from Mexican Chili Powder Reveals Growth Inhibition of Phytopathogenic Fungi.

strain Ch1 was isolated from Mexican chili powder, and the genome was sequenced. The genome was 4,765,544 bp in length, with an average G + C content of 56.22%, and a plasmid (pCh1) of 128,063 bp with an average G + C content of 52.

Global Situation of Bioremediation of Leachate-Contaminated Soils by Treatment with Microorganisms: A Systematic Review.

This systematic review presents the current state of research in the last five years on contaminants in soils, especially in leachates from solid waste landfills, with emphasis on biological remediation. In this work, the pollutants that can be treated by microorganisms and the results obtained worldwide were studied. All the data obtained were compiled, integrated, and analyzed by soil type, pollutant type, bacterial type, and the countries where these studies were carried out.

@magnetite Composite EDTA-Functionalized for the Potential Removal of Mercury.

spp. affects the Caribbean shores; thus, its remotion or valorization is a priority. This work aimed to synthesize a low-cost magnetically retrievable Hg adsorbent functionalized with ethylenediaminetetraacetic acid (EDTA) based on .

OxyR Positively Influences Phaseolotoxin Synthesis and Pyoverdin Production in pv. NPS3121.

Phaseolotoxin is a major virulence factor of the bean pathogen bacterium pv. . This toxin plays a key role in the development of the halo blight disease in bean plants.

Insights into the Bacterial Diversity and Detection of Opportunistic Pathogens in Mexican Chili Powder.

Chili powder is the most frequently consumed spice in Mexican diets. Thus, the dissemination of microorganisms associated with chili powder derived from L. is significant during microbial quality analysis, with special attention on detection of potential pathogens.

Detection of Isolates Posing Potential Health Risks in Mexican Chili Powder.

The potential presence of spore-forming bacteria related to the group in Mexican chili powder elaborated from L. is of commercial and clinical interest, because chili powder is an essential spice in the Mexican diet and in diets around the globe. To facilitate detection and isolation of members of this group of spore-forming bacteria from Mexican chili powder samples, we identified colonies that grew on agar medium selective for supplemented with polymyxin B (10 µg/mL) and ampicillin (10 to 100 µg/mL).

Effect of Salicylic Acid in the Yield of Ricinine in under Greenhouse Condition.

Castor bean () seeds contain ricinine, an alkaloid with insecticidal and insectistatic activities. Elicitation with salicylic acid (SA) has proven to stress and might modify the ricinine concentration. The aim of this study was to evaluate the concentration of ricinine in the bagasse of seeds from elicited with exogenous SA under greenhouse conditions.

Analysis of tRNA processing under salt stress in spore outgrowth using RNA sequencing data.

In spore-forming bacteria, the molecular mechanisms of accumulation of transfer RNA (tRNA) during sporulation must be a priority as tRNAs play an essential role in protein synthesis during spore germination and outgrowth. However, tRNA processing has not been extensively studied in these conditions, and knowledge of these mechanisms is important to understand long-term stress survival.    To gain further insight into tRNA processing during spore germination and outgrowth, the expression of the single copy tRNA gene was analyzed in the presence and absence of 1.

Rapid and reversible cell volume changes in response to osmotic stress in yeast.

Saccharomyces cerevisiae has evolved diverse mechanisms to osmotic changes: the cell wall, ion and water transport systems, and signaling cascades. At the present time, little is known about the mechanisms involved in short-term responses of osmotic stress in yeast or their physiological state during this process. We conducted studies of flow cytometry, wet weight measurements, and electron microscopy to evaluate the modifications in cell volume and the cell wall induced by osmotic stress.

Purpureocillium roseum sp. nov. A new ocular pathogen for humans and mice resistant to antifungals.

Background: Infectious keratitis is the main cause of preventable blindness worldwide, with about 1.5-2.0 million new cases occurring per year.

Richness and metallo-tolerance of cultivable fungi recovered from three high altitude glaciers from Citlaltépetl and Iztaccíhuatl volcanoes (Mexico).

In Mexico little is known about high-altitude glacial psychrotolerant or psychrophilic fungal species, with most glacial fungi isolated from polar environments or Alpine glaciers. It has been documented that some of these species may play an important role in bioremediation of contaminated environments with heavy metals. In the present study, 75 fungi were isolated from glaciers in Citlaltépetl (5675 masl) and Iztaccíhuatl (5286 masl) volcanoes.

Phylogenetic Analysis of Isolates from Commercial Bee Pollen Using tRNA-PCR.

Endospore-forming bacteria related to the group produce toxins that cause illnesses in organisms from invertebrates to mammals, including foodborne illnesses in humans. As commercial bee pollen can be contaminated with these bacteria, a comprehensive microbiological risk assessment of commercial bee pollen must be incorporated into the relevant regulatory requirements, including those that apply in Mexico. To facilitate detection of members of this group of bacteria, we have developed a PCR strategy that is based on the amplification of the single-copy tRNA gene and specific genes associated with tRNA to detect ().

Analysis of tRNA processing in the absence of CCAase in Bacillus subtilis.

In Bacillus subtilis, the tRNA lacks an encoded CCA 3' end. To gain insight into the role of CCAase and RNases in tRNA processing, several mutant strains were generated. Northern blot and RT-PCR results suggest that enzymes other than CCAase can participate in CCA addition at the 3' end of the immature tRNA.

Correction to: Evaluation of the presence of Paenibacillus larvae in commercial bee pollen using PCR amplification of the gene for tRNA.

In the article mentioned above an author's name was misspelled.

Bacterial Diversity Based on a 16S rRNA Gene Amplicon Data Set from a High-Altitude Crater Lake and Glacial Samples of the Iztaccihuatl Volcanic Complex (Mexico).

Little is known about extremophilic microorganisms from glaciers found in subtropical regions, and to our knowledge, no reports have identified glacial bacteria in this ecosystem in Mexico. Herein, we report a 16S rRNA gene amplicon data set demonstrating bacterial diversity of three samples from the Iztaccihuatl volcanic complex (Mexico) with a total of 115,701 to 138,805 high-quality reads. The bacterial population was classified at the phylum level in all samples.