Ultrafast detection of β-lactamase resistance in from blood culture by nanopore sequencing.

This study aimed to assess the ultra-fast method using MinION™ sequencing for rapid identification of β-lactamase-producing clinical isolates from positive blood cultures. Spiked-blood positive blood cultures were extracted using the ultra-fast method and automated DNA extraction for MinION sequencing. Raw reads were analyzed for β-lactamase resistance genes.