Immunogenicity of adenovirus and DNA vaccines co-expressing P39 and lumazine synthase proteins of Brucella abortus in BALB/c mice.

Brucella poses a great threat to animal and human health. Vaccination is the most promising strategy in the effort to control Brucella abortus (B. abortus) infection, but the currently used live vaccines interfere with diagnostic tests and could potentially result in disease outbreak.

Comparison of the adipogenesis in intramuscular and subcutaneous adipocytes from Bamei and Landrace pigs.

Fat deposition is a complex process involving proliferation, differentiation, and lipogenesis of adipocytes. Bamei and Landrace are considered to represent fat- and lean-type pig breeds. Subcutaneous (SC) and intramuscular (IM) pre-adipocytes were cultured to compare the proliferation and lipogenesis in these breeds.

[Studies on isolation, purification and antibacterial activities of beta-defensin in Yak neutrophils].

Aim: To isolate and purify beta-defensin from the neutrophils of Yak.

Methods: The method of percoll gradient centrifugation was employed to purify neutrophils from Yak peripheral blood. The crude extraction from Yak neutrophils was isolated respectively through a way of extraction by 50 mL/L acetic acid, acid soluble extract of Yak neutrophils was obtained.

[Establishing and identifying insulin resistance 3T3-L1 adipocytes cell model].

Objective: To establish insulin-resistant 3T3-L1 adipocytes cell model.

Methods: 3T3-L1 preadipocytes were cultured and induced to differentation. We established insulin resistant 3T3-L1 induced by dexamethasone and determined the change of glucose concentration in cell culture.