[Analysis of capability of examining helminthes: National technique competition of parasitic disease diagnosis in 2013].

Objectives: To understand the ability of worm detection of staff of centers for disease control and prevention (CDC) in China, so as to promote the ability construction.

Methods: In each province, four competitors were selected from the institutions of parasitic diseases control to attend the National Technique Competition for Parasitic Disease Diagnosis and the results of competition were collected and analyzed.

Results: The average scores of microscopic identification and slide preparation of all the 124 competitors were 27.

[Enterobius vermicularis infection status among children in 9 provinces/autonomous regions/municipalities of China].

Objective: To investigate the infection status of Enterobius vermicularis among children in 9 Provinces/Autonomous regions/Municipalities (P/A/M) of China, and analyze its risk factors.

Method: From April to December 2011, one provincial capital (prefecture-level city) and one county (city, district) were chosen as investigation spots from Guangdong, Guangxi, Hainan, Chongqing, Sichuan, Zhejiang, Fujian, Anhui and Guizhou, respectively. Children aged 2 to 12 were examined by using adhesive cellophane anal swab with round-bottom tube.

[Correlation between soil-transmitted nematode infections and children's growth].

Objective: To understand the infection status of soil-transmitted nematodes in southwest China and the correlation between soil-transmitted nematode infections and children's growth.

Methods: The prevalence of soil-transmitted nematode infections was determined by Kato-Katz technique, and in part of the children, the examination of Enterobius vermicularis eggs was performed by using the cellophane swab method. The influencing factors were surveyed by using a standardized questionnaire.

[Investigation on prevalence of soil-transmitted nematode infections and influencing factors for children in southwest areas of China].

Objective: To understand the infection status and main risk factors of soil-transmitted nematodes in southwest China so as to provide the evidence for making the control programs for soil-transmitted nematodiasis.

Methods: The prevalence of soil-transmitted nematode infections was determined by Kato-Katz technique and influencing factors were surveyed by using a standardized questionnaire, and in part of the children, the examination of Enterobius vermicularis eggs was performed by using the cellophane swab method. The relationship between soil-transmitted nematode infections and influencing factors was analyzed by the multiple probit estimated method.

[Cost-effectiveness analysis of integrated control strategy of parasitic diseases in demonstration plots].

Objective: To study the cost-effectiveness of different deworming schemes in demonstration plots of integrated control of parasitic diseases.

Methods: The cost-effectiveness was analyzed between mass drug administration and drug administration to focal population according to the different infection rates of parasites.

Results: In the demonstration plots of soil-born nematodes control, the costs for reducing one infected case and the cost for reducing 1% infection rate per ten thousand people in mass drug administration groups (Group 1 and Group 2) and drug administration to focal population were 20.

[Study on the mixed testing of serum samples in seroepidemiological survey of parasitic diseases].

Objective: In order to accumulate experiences for improving the efficiency in serological tests, the present study on mixed testing of serum samples was performed by taking the serological test of trichinellosis and toxoplasmosis as the examples, and had proved the effects on cost-effectiveness of seroepidemiological survey of parasitic disease with method of mixed-samples test.

Methods: According to the binomial distribution principle, to develop an approach to the feasibility of mixed testing of serum samples, and to work on a cost-effectiveness analysis of one-by-one testing and mixed testing using hygienic economic analysis method was performed. For serological test of trichinellosis and toxoplasmosis, 3 kinds of mixed testing methods, namely 3 serum sample mixture, 5 serum sample mixture and 10 serum sample mixture, were performed.

New perspectives on host-parasite interplay by comparative transcriptomic and proteomic analyses of Schistosoma japonicum.

Schistosomiasis remains a serious public health problem with an estimated 200 million people infected in 76 countries. Here we isolated ~ 8,400 potential protein-encoding cDNA contigs from Schistosoma japonicum after sequencing circa 84,000 expressed sequence tags. In tandem, we undertook a high-throughput proteomics approach to characterize the protein expression profiles of a number of developmental stages (cercariae, hepatic schistosomula, female and male adults, eggs, and miracidia) and tissues at the host-parasite interface (eggshell and tegument) by interrogating the protein database deduced from the contigs.

Rapid kinetic rate assay of the serum alpha-L-fucosidase in patients with hepatocellular carcinoma by using a novel substrate.

Background: Developing kinetic rate assay kit for alpha-L-fucosidase (AFU) by using a novel substrate 2-chloro-4-nitrophenyl-alpha-L-fucopyranoside (CNPF) and clinical implication in the diagnosis of hepatocellular carcinoma (HCC).

Methods: We have evaluated the new kinetic rate assay kit using a novel substrate for serum AFU employing Hitachi 7170 automated analyzer. The reaction was carried out at 37 degrees C, monitoring the wavelength at 405 nm.

Evolutionary and biomedical implications of a Schistosoma japonicum complementary DNA resource.

Schistosoma japonicum causes schistosomiasis in humans and livestock in the Asia-Pacific region. Knowledge of the genome of this parasite should improve understanding of schistosome-host interactions, biomedical aspects of schistosomiasis and invertebrate evolution. We assigned 43,707 expressed sequence tags (ESTs) derived from adult S.

[Prokaryotic expression of gene encoding Schistosoma japonicum SjE16 and its potential application in immunodiagnosis].

Objective: To sub-clone and express the gene encoding Schistosoma japonicum calcium-binding protein (SjE16) and study its immunological response.

Methods: The specific primers were designed according to the expressed sequence tags (ESTs) sequence, which was used for amplification of the encoding sequence from the cDNA clone containing SjE16. The gene was subcloned into pGEX4T-1 plasmid and expressed.