[Establishment of a Patient-Derived T-Cell Acute Lymphoblastic Leukemia Xenograft Model in Novel Immunodeficient NCG Mice].

Objective: The leukemia cells from patients with T-cell acute lymphoblastic leukemia (T-ALL) were inoculated into NCG mice to establish a stable human T-ALL leukemia animal model.

Methods: Leukemia cells from bone marrow of newly diagnosed T-ALL patients were isolated, and the leukemia cells were inoculated into NCG mice via tail vein. The proportion of hCD45 positive cells in peripheral blood of the mice was detected regularly by flow cytometry, and the infiltration of leukemia cells in bone marrow, liver, spleen and other organs of the mice was detected by pathology and immunohistochemistry.

Parallel subgenome structure and divergent expression evolution of allo-tetraploid common carp and goldfish.

How two subgenomes in allo-tetraploids adapt to coexistence and coordinate through structure and expression evolution requires extensive studies. In the present study, we report an improved genome assembly of allo-tetraploid common carp, an updated genome annotation of allo-tetraploid goldfish and the chromosome-scale assemblies of a progenitor-like diploid Puntius tetrazona and an outgroup diploid Paracanthobrama guichenoti. Parallel subgenome structure evolution in the allo-tetraploids was featured with equivalent chromosome components, higher protein identities, similar transposon divergence and contents, homoeologous exchanges, better synteny level, strong sequence compensation and symmetric purifying selection.

Optimum dredging time for inhibition and prevention of algae-induced black blooms in Lake Taihu, China.

Dredging, which is the removal of polluted surface sediments from a water body, is an effective means of preventing the formation of algae-induced black blooms. However, an inappropriate dredging time may contribute to rather than inhibit the formation of black blooms. To determine the optimum dredging time, four treatments were simulated with sediment samples collected from Lake Taihu: dredging in January 2014 (DW), April 2014 (DA), July 2014 (DS), and no dredging (UD).

[Environmental Effect of Substrate Amelioration on Lake: Effects on Phragmites communis Growth and Photosynthetic Fluorescence Characteristics].

Growth of rooted aquatic macrophytes was affected by the nature and composition of lake bottom sediments. Obviously, it has been recognized as an important ecological restoration measure by improving lake substrate and then reestablishing and restoring aquatic macrophytes in order to get rid of the environmental problem of lake. This study simulated five covering thickness to give an insight into the influence of substrate amelioration on Phragmites communis growth and photosynthetic fluorescence characteristics.

[Simulation research on the release of internal nutrients affected by different dredging methods in lake].

A simulated experiment was carried out to study release features of internal source under different sediment dredging methods and the difference between two lake areas in Lake Taihu was also studied. The contaminated sediments were sampled from two sites in Meiliang Bay which were the Inner Bay (A) and the Outer Bay(B). Release rates of phosphorus after ideal dredging and suction dredging are about 20% and 72% of the control and the phosphorus release rate in Inner Bay(A) is about 80% of Outer Bay(B).

[Sequences analysis of jlFABP2 and the correlation between polymorphisms and body weight gain in Cyprinus carpio var. jian].

Two replicate intestine fatty acid binding protein genes (jlFABP2a and jlFABP2b) were cloned from Cyprinus carpio var. jian using PCR. Both ORFs were 399 bp in length sharing 92.

Two Δ6-desaturase-like genes in common carp (Cyprinus carpio var. Jian): structure characterization, mRNA expression, temperature and nutritional regulation.

Δ6-Desaturase is the rate-limiting enzyme involved in highly unsaturated fatty acid (HUFA) biosynthesis. There is very little information on the evolution and functional characterization of Δ6Fad-a and Δ6Fad-b in common carp (Cyprinus carpio var. Jian).

[Influence of dredging on sediment resuspension and phosphorus transfer in lake: a simulation study].

A simulated experiment was conducted to investigate the impacts of sediment dredging on sediment resuspension and phosphorus transfer in the summer and winter seasons under the common wind-wave disturbance, and the contaminated sediment used in this study was from Meiliang Bay, Taihu lake. The result showed that 20 cm dredging could effectively inhibit the sediment resuspension in study area, dredging in winter has a better effect than that in summer, and the higher values of the total suspended solid (TSS) in undredged and dredged water column during the process of wind wave disturbance were 7.0 and 2.

[Microsatellite markers for parentage identification in Jian Carp (Cyprinus carpio var. Jian)].

Using 16 microsatellite loci we estimated the parentage of 647 progeny in 10 Jian Carp full-sib families. Cervus 3.0 analysis showed that mean PIC value of 16 microsatellites, mean number of allele, and mean expected heterozygosity were 0.

Identification of housekeeping genes suitable for gene expression analysis in Jian carp (Cyprinus carpio var. jian).

Jian carp (Cyprinus carpio var. jian) is an important economic fish species cultured in China. In this report, we performed a systematic analysis to identify an appropriate housekeeping (HK) gene for the study of gene expression in Jian carp.

Influence of dietary fatty acids on muscle fatty acid composition and expression levels of Δ6 desaturase-like and Elovl5-like elongase in common carp (Cyprinus carpio var. Jian).

The effects of dietary fatty acids on muscle fatty acid composition and liver expression levels of Δ6 desaturase-like and Elovl5-like elongase were investigated in common carp (Cyprinus carpio var. Jian). Two Δ6 desaturase-like cDNAs (Fad6-a and Fad6-b) and two Elovl5-like elongase (Elovl5-a and Elovl5-b) cDNAs were cloned.

Cloning, structure, and expression pattern of the P-450 aromatase gene in rice field eel (Monopterus albus).

We report the cloning, tissue expression, and structural analysis of the aromatase gene in the rice field eel (Monopterus albus). The ovary-derived cDNA (1,802 bp) has a 49 bp 5'-untranslated region (UTR), a 202 bp 3'-UTR, and a 1,551 bp open-reading frame, which encodes a protein of 517 amino acid residues with a predicted molecular weight of 58.2 kDa.