Efficient Secretion of Recombinant Proteins from Rice Suspension-Cultured Cells Modulated by the Choice of Signal Peptide.

Plant-based expression systems have emerged as a competitive platform in the large-scale production of recombinant proteins. By adding a signal peptide, αAmy3sp, the desired recombinant proteins can be secreted outside transgenic rice cells, making them easy to harvest. In this work, to improve the secretion efficiency of recombinant proteins in rice expression systems, various signal peptides including αAmy3sp, CIN1sp, and 33KDsp have been fused to the N-terminus of green fluorescent protein (GFP) and introduced into rice cells to explore the efficiency of secretion of foreign proteins.

Source characterization of total suspended particulate matter near a riverbed in Central Taiwan.

Samples of total suspended particulate (TSP) matter were collected by using TSP samplers from certain areas representing the estuary of the Jhuoshuei River, Taiwan during monsoon and non-monsoon seasons in 2005. A total of 12 elements and nine inorganic ions were identified by inductively coupled plasma atomic emission spectrometry (ICP-AES) and ion chromatography (IC), respectively. Enrichment factors explaining a preponderance of the variance in the data were applied to the data sets.