Effects of three-area laser-assisted zona thinning in 8-cell human embryos on pregnancy outcomes fertilization.

Objective: This study conducted a preliminary examination of the effects of three-area laser-assisted zona thinning (LAZT) during the cleavage stage of embryo development on the hatching process in human fertilization-embryo transfer (IVF-ET) with subjects of advanced female age or frozen-thawed (FT) embryos.

Methods: Eight-cell stage embryos were treated with LAZT in three areas of the zona pellucida at 120° intervals. The control group was embryos without LAZT.

Quantitative Assessment of Endometrial Volume and Uterine Vascularity and Pregnancy Outcome in Frozen-Thawed Embryo Transfer Cycles.

Objective: To investigate the usefulness of the endometrial volume and vascular indices in the endometrial region as an effective predictor for pregnancy outcome in frozen-thawed embryo transfer (FET).

Study Design: We evaluated 131 embryo transfer cycles in 73 infertile women. After controlled ovarian stimulation all embryos were cultured to blastocyst stage, and the blastocysts with good quality were vitrified for elective FET.

Vitrification solution without sucrose for cryopreservation in mouse blastocysts.

Objective: This study was designed to investigate the survival rate of vitrified mouse blastocysts depending on the presence or absence of sucrose in vitrification solution.

Methods: Mouse two-cell embryos were collected and cultured to blastocysts. Two vitrification solutions were prepared.

Measurement of endometrial and uterine vascularity by transvaginal ultrasonography in predicting pregnancy outcome during frozen-thawed embryo transfer cycles.

Aim: An appropriate endometrial condition and vascular supply are usually considered essential for implantation of an embryo. This study was performed to assess the role of endometrial and uterine vascularity status measurement in predicting pregnancy outcome during frozen-thawed embryo transfer cycles.

Material And Methods: A total of 70 infertile women were recruited with controlled ovarian stimulation followed by oocyte retrieval.

Effects of methimazole on the onset of type 2 diabetes in leptin receptor-deficient rats.

We investigated the effects of methimazole, an anti-thyroid drug, on the onset of type 2 diabetes in Zucker diabetic fatty (ZDF) rats. For this, 0.03% methimazole was administered to 7-week-old, pre-diabetic ZDF rats in drinking water for 5 weeks and the animals were sacrificed at 12 weeks of age.