Publications by authors named "Ju Yeon Lee"
Metformin (MetF) is used worldwide as a first-line therapy for type 2 diabetes. Recently, interest in the pleiotropic effects of MetF, such as its anticancer and antiaging properties, has increased. However, the molecular target of MetF and the detailed mechanism underlying its ability to inhibit cell growth through autophagy induction remain incompletely understood.
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- - The glycosylation profile of therapeutic proteins, especially sialylation, is important for their effectiveness and stability, particularly in fusion proteins used to treat angiogenic disorders.
- - A mass spectrometry method was used to analyze sialylation levels in VEGFR-IgG fusion proteins by examining different fractions from production media, revealing varying sialylation levels.
- - The study found that the overall sialylation levels matched the expected results from the different fractions, emphasizing the importance of LC-MS/MS-based analysis for quality control and consistency in biosimilar development.
Article Synopsis
- This longitudinal study analyzed how serum Brain-Derived Neurotrophic Factor (sBDNF) levels and childhood adversities affect the development of Post-Traumatic Stress Disorder (PTSD) in individuals with physical injuries over two years.
- Researchers assessed patients at a trauma center for sBDNF levels and childhood adversities using the ACE questionnaire and diagnosed PTSD at multiple intervals employing the Clinician-Administered PTSD Scale.
- Results showed that lower sBDNF levels increased the risk of earlier-onset PTSD among those who experienced childhood adversities, highlighting the importance of both biological and environmental factors in PTSD development.
Article Synopsis
- * Out of 538 participants, 58 (10.8%) developed PTSD, with rates decreasing over time from 8.4% at 3 months to 2.5% at 24 months.
- * Results showed that lower SDNN was linked to PTSD in patients who did not undergo surgery, suggesting that PTSD prevention strategies should take both SDNN levels and surgical status into account.
We investigated the regulation of histone deacetylases (HDACs) by miR-2861 in the osteoblastic differentiation of human mesenchymal stem cells (MSCs) and miR-2861 binding site by CRISPR activation (CRISPRa). Transfection of miR-2861 into human MSCs was performed and the effect on osteoblast differentiation was analyzed. Using catalytically inactive Cas12a, the CRISPRa system induced targeted overexpression of endogenous miRNA and repressed the luciferase activities of reporters that contained functional miRNA target sites.
View Article and Find Full Text PDFBackground: This study investigated the longitudinal associations between serum BDNF (sBDNF) levels measured early after injury and the onset of post-traumatic stress disorder (PTSD) across two years.
Methods: Patients with moderate to severe physical injuries were enrolled from a trauma center. At baseline, sBDNF levels were measured and a comprehensive socio-demographic and clinical data were collected.
Biomarkers for predicting depression after acute coronary syndrome.
Gen Hosp Psychiatry
September 2024
Imaging-based spatial transcriptomics technologies such as Multiplexed error-robust fluorescence in situ hybridization (MERFISH) can capture cellular processes in unparalleled detail. However, rigorous and robust analytical tools are needed to unlock their full potential for discovering subcellular biological patterns. We present Intracellular Spatial Transcriptomic Analysis Toolkit (InSTAnT), a computational toolkit for extracting molecular relationships from spatial transcriptomics data at single molecule resolution.
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- The Clinician-Administered PTSD Scale (CAPS-5) is a reliable tool for diagnosing PTSD and was used in this study to assess its predictive value for PTSD development in patients with moderate to severe physical injuries over a 2-year period.
- 1,142 patients were evaluated for acute stress disorder at baseline, and follow-up assessments were conducted at multiple intervals using CAPS-5 to track PTSD development.
- Results indicated that while baseline CAPS-5 diagnoses showed limited predictive ability, scores of 15 or higher effectively predicted later PTSD, with scores of 16 or higher further increasing accuracy, especially in patients with intentional injuries or prior trauma.
Purpose: The immunogenicity of vaccines containing the canine adenovirus (CAdV) type 2 (CAdV-2) variant has not yet been reported. We prepared a novel inactivated CAdV-2 variant vaccine using the CAV2232-41 strain, and evaluated its safety and immunogenicity in raccoon dogs.
Materials And Methods: The growth kinetics of CAV2232-41 were determined using Madin-Darby Canine Kidney (MDCK) cells.
Article Synopsis
- The study investigated how serum cortisol levels and personality traits impact the risk of developing PTSD over two years in individuals with physical injuries.
- It involved tracking 923 patients from a trauma center, measuring their cortisol levels and personality traits, while diagnosing PTSD at multiple intervals, showing that overall PTSD rates decreased over time.
- A significant finding revealed that lower cortisol levels combined with higher Neuroticism increased PTSD risk, especially in the early months, highlighting the complex relationship between biological factors and personality in PTSD development.
Delayed effects of alcohol consumption on the association between serum BDNF levels and post-traumatic stress disorder development over two-years.
Prog Neuropsychopharmacol Biol Psychiatry
December 2024
Article Synopsis
- This study investigated how serum BDNF (sBDNF) levels and alcohol consumption status affect the risk of developing PTSD after a physical injury over a two-year period.
- It involved 923 trauma center participants, with 12.1% developing PTSD, revealing that lower sBDNF levels were linked to PTSD in alcohol consumers, but not in non-drinkers.
- The findings suggest a crucial interaction between sBDNF and alcohol consumption in predicting PTSD, emphasizing the importance of considering these factors in prevention strategies, although the study’s focus on a single trauma center may affect overall applicability.
Article Synopsis
- Increased fast food consumption among adolescents during COVID-19 school closures is linked to poor dietary and lifestyle choices, raising public health concerns.
- A study involving 1,710 middle and high school students in Gwangju, South Korea found that factors like irregular meal patterns, decreased physical activity, and increased sedentary behavior contributed to this trend.
- The findings emphasize the need for dietary interventions and guidelines to promote healthier eating habits among adolescents during times of disruption, like school closures.*
Objectives: To evaluate the cost-effectiveness of the 20-valent pneumococcal conjugate vaccine (PCV20) compared to 13-valent pneumococcal conjugate vaccine (PCV13) for the pediatric population in Korea, where the four-dose vaccine coverage rate is over 97%.
Methods: We constructed a Markov model to calculate the cost and quality-adjusted life-years (QALYs) over 10 years. The health states were susceptible states; disease states, which included invasive pneumococcal diseases such as meningitis, bacteremia, pneumonia, and acute otitis media; and death attributable to pneumococcal disease.
Article Synopsis
- - The study explored factors that predict early-onset (within 6 months) and delayed-onset (after 6 months) PTSD in patients following physical injuries over two years.
- - Data was collected from 1142 patients in South Korea, assessing various personal, trauma-related, and psychological factors, with diagnoses made using the Clinician-administered PTSD Scale at multiple follow-up points.
- - Results showed that early-onset PTSD was more likely in individuals with previous psychiatric issues and higher anxiety, while delayed-onset PTSD was associated with greater injury severity and life stressors, highlighting the need for tailored interventions.
Article Synopsis
- * Results showed a significant increase in both depression (mean score increased from 2.37 to 5.39) and anxiety (mean score increased from 1.41 to 3.41) among healthcare workers, with those experiencing moderate to severe conditions rising dramatically post-pandemic.
- * The findings highlight the need for ongoing monitoring of healthcare workers’ mental health, emphasizing the importance of social support and strategies to alleviate their workload and psychological stress during health crises.