Publications by authors named "Jozef J M Van der Steen"

Environmental monitoring is crucial for assessing the overall state of the ecosystems in terms of contaminant impact and chemical landscape. The use of honey bee (Apis mellifera) colonies considerably eases the sampling activities, as honey bees are exposed to a wide range of substances that are transported and accumulated within the beehives. In this work, combining low-resolution and high-resolution mass spectrometry, the APIStrip passive sampler has been employed to evaluate the presence of pesticide residues and the overall characterization of beehive environments.

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Recent research has highlighted the potential of honeybees and bee products as biological samplers for monitoring xenobiotic pollutants. However, the effectiveness of these biological samplers in tracking microplastics (MPs) has not yet been explored. This study evaluates several methods of sampling MPs, using honeybees, pollen, and a novel in-hive passive sampler named the APITrap.

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The steady conditions of temperature, humidity and air flux within beehives make them a valuable location for conducting environmental monitoring of pollutants such as PAHs. In this context, the selection of an appropriate apicultural matrix plays a key role in these monitoring studies, as it maximizes the information that will be obtained in the analyses while minimizing the inaccurate results. In the present study, three apicultural matrices (honey bees, pollen and propolis) and two passive samplers (APIStrips and silicone wristbands) are compared in terms of the number and total load of PAHs detected in them.

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Western honey bees are very sensitive bioindicators for studying environmental conditions, hence frequently included in many investigations. However, it is very common in both research studies and health surveillance programs to sample different components of the colony, including adult bees, brood and their food reserves. These practices are undoubtedly aggressive for the colony as a whole, and may affect its normal functioning and even compromise its viability.

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The use of apicultural matrices for the environmental monitoring of pesticides is a widely employed approach that facilitates to a great extent the sampling procedures. Honey bees are one of the most commonly employed matrices in these studies due to their abundance in the colonies and their direct contact with the beehive and the environment. However, the analysis of this matrix is associated to a lack of representativity of the contaminants accumulated within the beehive, due mainly to the limited number of honey bees that are sampled and analyzed compared to the population in a hive.

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A diverse supply of pollen is an important factor for honey bee health, but information about the pollen diversity available to colonies at the landscape scale is largely missing. In this COLOSS study, beekeeper citizen scientists sampled and analyzed the diversity of pollen collected by honey bee colonies. As a simple measure of diversity, beekeepers determined the number of colors found in pollen samples that were collected in a coordinated and standardized way.

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Due to their extensive use in both agricultural and non-agricultural applications, pesticides are a major source of environmental contamination. Honey bee colonies are proven sentinels of these and other contaminants, as they come into contact with them during their foraging activities. However, active sampling strategies involve a negative impact on these organisms and, in most cases, the need of analyzing multiple heterogeneous matrices.

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The active substances coumaphos, tau-fluvalinate and amitraz are among the most commonly employed synthetic miticides to control varroa infestations in apiculture. These compounds can persist inside the beehive matrices and can be detected long time after their application. The present study describes the application of a new passive sampling methodology to assess the dissipation of these miticides as well as the cross-contamination in neighboring beehives.

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Microplastics are ubiquitous and their sampling is a difficult task. Honeybees interact with the environment inside their foraging range and take pollutants with them. In this work, we demonstrated for the first time that worker bees can act as active samplers of microplastics.

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Honeybee colonies are proven bio-samplers in their foraging area, as organic contaminants such as pesticides are continuously deposited in their hives. However, the use of honeybee colonies for the biomonitoring of contaminants requires the sampling of biological matrices such as bees, pollen, honey or beeswax. This active sampling alters the colonies, especially in the case of frequent sampling intervals.

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Article Synopsis
  • The study developed a mechanistic model to understand honeybee foraging behavior in relation to pesticide exposure across varied landscapes, focusing on how bees choose resource patches based on energy efficiency.
  • The model simulates strategies for patch selection—including selecting a single optimal patch or exploiting multiple patches simultaneously—and considers factors like resource availability and competition among foragers throughout the day.
  • By using this model, researchers assessed how different landscape compositions could dilute pesticide exposure in honeybee foraging, ultimately providing a framework for improving landscape management to protect bees.
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Background: Recent elevated winter loss of honey bee colonies is a major concern. The presence of the mite Varroa destructor in colonies places an important pressure on bee health. V.

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Honeybees (Apis mellifera L.) have great potential for detecting and monitoring environmental pollution, given their wide-ranging foraging behaviour. Previous studies have demonstrated that concentrations of metals in adult honeybees were significantly higher at polluted than at control locations.

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To evaluate the effect of the indoxacarb 300 g kg(-1) WG, Steward 30WDG, on the honey bee (Apis mellifera L.) in apple orchards, a monitoring study was conducted in Dutch apple orchards in April/May 2004. Before apple flowering began, two honey bee colonies were placed in each orchard to investigate honey bee mortality.

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