Time Course of Inflammatory Gene Expression Following Crush Injury in Murine Skeletal Muscle.

Background: Early inflammation and secretion of proinflammatory cytokines such as IL-1β, IL-6, and TNF-α act as the key drivers to regulate inflammation after muscle injury. However, the effects of these key proinflammatory drivers in a noninvasive crush injury model are not well known. Understanding these effects is important for treating crush injuries that occur during natural disasters and military conflicts.

Evaluation of cellular wound healing using flow cytometry and expanded polytetrafluroethylene implants.

In wound healing studies that investigate therapeutic interventions, it is important to characterize cellular responses. In a randomized trial enrolling patients at risk for surgical infection, one goal is to phenotype cells within a polytetrafluoroethylene implant using flow cytometry and immunohistochemistry, together with standard hematoxylin- and eosin-based histology. Subcutaneous implants are removed 8-9 days postoperatively.