QTL analysis of sex pheromone blend differences between two closely related moths: Insights into divergence in biosynthetic pathways.

To understand the evolution of premating signals in moths, it is important to know the genetic basis of these signals. We conducted Quantitative Trait Locus (QTL) analysis by hybridizing two noctuid moth species, Heliothis virescens (Hv) and Heliothis subflexa (Hs), and backcrossing the F(1) females to males of both parental species. One of these backcrosses (F(1) x Hs) was a biological replicate of our previous study (Sheck et al.

Experimental evidence for interspecific directional selection on moth pheromone communication.

The chemical composition of the sexual communication signals of female moths is thought to be under strong stabilizing selection, because females that produce atypical pheromone blends suffer lower success in finding mates. This intraspecific selection pressure cannot explain the high diversity of moth pheromone blends found in nature. We conducted experiments to determine whether communication interference from males of closely related species could exert strong enough directional selection to cause evolution of these signals.