Tylosin A and desmycosin in honey by salting-out assisted liquid-liquid extraction and aqueous normal phase ultraperformance liquid chromatography-tandem mass spectrometry.

A simple and rapid method was developed for the determination of tylosin A and desmycosin residues in honey. Aliquots of honey samples were dissolved in a concentrated solution of sodium acetate and the target analytes were subsequently extracted with acetonitrile. The resulting organic extract was chromatographed under aqueous normal phase (ANP) LC conditions using a bare silica stationary phase with acidified solutions of ammonium formate in both water and 5:95 water: acetonitrile as the mobile phases.