In vitro effect of beer, red and white wine on the morphology and surface roughness of human enamel.

Background: Beer, red and white wine are acidic drinks whose frequent consumption can increase the risk of dental erosion.

Objectives: To establish the effect of beer, red and white wine on the morphology and surface roughness (SR) of human enamel using different exposure times in a cyclic deand remineralization model in vitro.

Material And Methods: The experiment included 33 surgically extracted impacted human third molars from patients aged 18-25 years.

Ultrastructural changes in the cemento-enamel junction caused by acidic beverages: An in vitro study.

The present in vitro study was aimed at evaluating the morphological changes in the cemento-enamel junction (CEJ) after exposure to acidic beverages using the scanning electron microscopy (SEM). The initial pH and titratable acidity (TA) was analyzed from follow groups: (I) Coca cola, (II) orange juice, (III) Cedevita, (IV) Red Bull, (V) Somersby cider, and (VI) white wine. The CEJ samples (n = 64), obtained from unerupted third molars, were allocated to one control (artificial saliva, n = 16) and six experimental groups (n = 8).

Prevalence of Human Cytomegalovirus and Epstein-Barr Virus in Chronic Periapical Lesions.

Objective: The aim of this study was to investigate the presence of human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) in the tissue of chronic periapical lesions, and to compare the results in relation to the symptoms of patients and the size of the lesion.

Methods: Periapical lesions analyzed in the study were collected from the roots of the teeth indicated for extraction. Samples were divided according to the symptoms into groups of symptomatic and asymptomatic, and according the size into groups of small and large lesions.

Erosive Effect of Different Soft Drinks on Enamel Surface in vitro: Application of Stylus Profilometry.

Objective: To assess the erosive potential of various soft drinks by measuring initial pH and titratable acidity (TA) and to evaluate enamel surface roughness using different exposure times.

Materials And Methods: The initial pH of the soft drinks (group 1: Coca-Cola; group 2: orange juice; group 3: Cedevita; group 4: Guarana, and group 5: strawberry yoghurt) was measured using a pH meter, and TA was measured by titration with NaOH. Enamel samples (n = 96), cut from unerupted human third molars, were randomly assigned to 6 groups: experimental (groups 1-5) and control (filtered saliva).

[Analysis of antimicrobial effect of MTAD solution in infected root canal system using PCR technique].

Introduction: Clinically acceptable antiseptic should possess organolithic-mineralolithic properties and antimicrobial efficacy, and should be non-toxic.

Objective: The aim of the paper was to assess the presence of genomes of the most common microorganisms (Porphyromonas gingivalis, Agregatibacter actinomycetemcomitans, Tanerella forsythensis, Prevotella intermedia, Treponema denticola and Enterococcus faecalis) in infected tooth root canals before and after rinsing with solution of doxycycline, citric acid and detergent Tween-80 (MTAD) in patients with clinically diagnosed primary apex periodontitis.

Methods: The content of primarily infected canals before and after using the MTAD solution was used as a biological material in which the presence of microorganisms DNA was proved.

Ultrastructural analysis of the root canal walls after simultaneous irrigation of different sodium hypochlorite concentration and 0.2% chlorhexidine gluconate.

To determine whether sodium hypochlorite (NaOCl) with 0.2% chlorhexidine gluconate (CHX) leads to colour change and precipitate formation, and to ultrastructurally analyse the dentine surface after simultaneous irrigation with 0.5% NaOCl and 0.

Ultrastructural changes in the cemento-enamel junction after vital tooth bleaching with fluoride and fluoride-free agents - a pilot study.

Background: The impact of bleaching on the cemento-enamel junction (CEJ) is not well known. Due to frequent sensitivity of the cervical region of teeth after the vital bleaching, the aim of the present study was to evaluate the morphological features of the CEJ of human teeth after application of fluoridated and fluoride-free bleaching agents, as well as post-bleaching fluoridation treatment, by scanning electron microscopy (SEM) analysis.

Material/methods: Thirty-five extracted permanent human teeth were longitudinally cut, yielding 70 specimens.

[Diabetes mellitus and periodontal disease].

Introduction: This review highlights the relations between diabetes mellitus and periodontal disease, as well as the pathogenic mechanisms which are still the subject of investigations. The possible pathogenic mechanisms important for developing periodontal disease in the diabetics are: vascular gingival changes, the disorders in the metabolism of the collagen, disorders in the function of the polimorphonuclear leukocytes and specific microbial flora in the parodontal pockets.

Conclusion: It is clear that adequate early recognition and good treatment are very important and significant for treatment of diabetic periodontal disease.

[The investigation of ultrasound efficacy in cleaning the surface of new endodontic instruments].

Introduction: Active parts and tips of various new stainless steel and nickel-titanium endodontic instruments can be coated with fragments or metal residues, which can become detached during endodontic treatment. These fragments may obstruct the root canals or even reach the periapical tissue during biomechanical preparation and should be removed before clinical use.

Objective: The aim of this study was to evaluate the presence of metal residues on both new stainless steel and nickel-titanium endodontic instruments, and to determine the cleaning efficacy of ultrasound using distilled water or disinfectant solution for removing these residues.