Publications by authors named "Josua Schinke"
DenA/DEN1 and the COP9 signalosome (CSN) represent two deneddylases which remove the ubiquitin-like Nedd8 from modified target proteins and are required for distinct fungal developmental programmes. The cellular DenA/DEN1 population is divided into a nuclear and a cytoplasmatic subpopulation which is especially enriched at septa. DenA/DEN1 stability control mechanisms are different for the two cellular subpopulations and depend on different physical interacting proteins and the C-terminal DenA/DEN1 phosphorylation pattern.
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- Deneddylases remove the Nedd8 protein from other proteins, and increased activity of these enzymes is linked to cancer in humans.
- A mutant strain of Aspergillus nidulans lacking two specific deneddylases shows reduced growth and multicellular development, indicating that these enzymes are essential for proper fungal development.
- The interaction between the DEN1/DenA deneddylase and the COP9 signalosome affects protein degradation and highlights the importance of balanced deneddylase activity for healthy multicellular growth in fungi.
Defects in the COP9 signalosome (CSN) impair multicellular development, including embryonic plant or animal death or a block in sexual development of the fungus Aspergillus nidulans. CSN deneddylates cullin-RING ligases (CRLs), which are activated by covalent linkage to ubiquitin-like NEDD8. Deneddylation allows CRL disassembly for subsequent reassembly.
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