Isolation of Bioactive Compounds from Calicotome villosa Stems.

A phenylethanoid, two steroids, a flavone glucoside and a chalcone have been isolated for the first time from the stems of together with a previously isolated flavone glucoside. Their structures were determined by spectroscopic analyses (NMR, HRMS) as basalethanoïd B (), β-sitosterol and stigmasterol (), chrysine-7--β-d-glucopyranoside (), chrysine 7-((6''--acetyl)--β-d-glucopyranoside) () and calythropsin (). The crude extracts and the isolated compounds (except 4), were evaluated for their antioxidant, antimicrobial (against two Gram-positive bacterial strains: , , four Gram-negative bacterial strains: , , , and three yeasts: , , and ), hemolytic, antidiabetic, anti-inflammatory and cytotoxic activity.