Publications by authors named "Joshua N Brehm"
YabG is a sporulation-specific protease that is conserved among sporulating bacteria. Clostridioides difficile YabG processes the cortex destined proteins preproSleC into proSleC and CspBA to CspB and CspA. YabG also affects synthesis of spore coat/exosporium proteins CotA and CdeM.
View Article and Find Full Text PDFClostridioides difficile is a pathogen whose transmission relies on the formation of dormant endospores. Spores are highly resilient forms of bacteria that resist environmental and chemical insults. In recent work, we found that C.
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- Taking antibiotics can mess up the healthy bacteria in our gut, which might make it easier for infections to happen.
- The study looked at how different antibiotics changed the bacteria in healthy people's intestines and found that different antibiotics affected them in different ways.
- Surprisingly, some changes in bacteria and bile salts didn’t necessarily mean a higher chance of infection, showing there are other ways bacteria can control infections without relying just on bile salts.
YabG is a sporulation-specific protease that is conserved among sporulating bacteria. YabG processes cortex destined proteins preproSleC into proSleC and CspBA to CspB and CspA. YabG also affects synthesis of spore coat/exosporium proteins CotA and CdeM.
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- Clostridioides difficile, a non-model enteropathogenic bacterium, presents challenges for targeted mutagenesis, which is essential for studying its disease-causing mechanisms.
- Traditional mutagenesis methods, including uracil auxotrophy and CRISPR/Cas, have their own limitations, making mutation generation difficult.
- The study introduces a new allelic exchange strategy using a theophylline-dependent riboswitch to improve mutation screening and selection, allowing for efficient mutant creation without the drawbacks of other methods.
is a pathogen whose transmission relies on the formation of dormant endospores. Spores are highly resilient forms of bacteria that resist environmental and chemical insults. In recent work, we found that SspA and SspB, two small acid-soluble proteins (SASPs), protect spores from UV damage and, interestingly, are necessary for the formation of mature spores.
View Article and Find Full Text PDFPlasmid Sequence and Availability for an Improved Clostridioides difficile CRISPR-Cas9 Mutagenesis System.
Microbiol Resour Announc
December 2022
A two-plasmid mutagenesis system for Clostridioides difficile is described that improves ease of use and efficiency in creating site-directed mutations. pJB06 contains a xylose-inducible gene, while the second plasmid (pJB07) encodes the corresponding guide RNA (gRNA) and regions of homology for repair of the introduced double-stranded DNA (dsDNA) breaks, both of which are replaceable via restriction digest.
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