Publications by authors named "Joshua Galloway"
Purpose: We evaluate the quality and feasibility of preloading Descemet stripping automated endothelial keratoplasty (DSAEK) grafts into a modified EndoGlide Ultrathin system for graft injection.
Methods: DSAEK grafts were prepared by experienced processing technicians at 2 separate locations, loaded into a modified EndoGlide Ultrathin, and placed in storage media. Grafts processed at one location were shipped cross-country overnight to the other location and were examined on arrival for positioning within the modified EndoGlide Ultrathin.
Article Synopsis
- Multiple myeloma (MM) is a type of cancer involving abnormal plasma cells with various genetic changes that affect DNA repair and response mechanisms.
- These alterations allow cancers to grow, evolve, and resist treatment, often due to the malfunction of key genes like TP53 and TP73, which normally help manage damaged DNA.
- The review focuses on how these DNA damage response systems operate in healthy cells versus in MM, as well as discussing treatment strategies that leverage this knowledge for better patient outcomes.
Purpose: The aim of this study was to compare the performance of Kerasave and Optisol-GS for hypothermic corneal storage for 14 days.
Methods: This study was a prospective laboratory investigation. Mate corneas were recovered into Kerasave or Optisol-GS (27 pairs) and stored at 2°C to 8°C for 14 days.
Purpose: To investigate stamp visibility and endothelial cell loss (ECL) after the application of an orientation mark to Descemet membrane endothelial keratoplasty (DMEK) grafts supported by an air bubble.
Methods: Eighteen DMEK grafts were prepared at an eye bank using a technique where an orientation mark was applied to the stromal surface of a DMEK graft that was supported by a small air bubble placed at the edge of the 2 endothelial surfaces of the graft. Grafts were evaluated at 2 and 5 days for stamp visibility and at 5 days with calcein-AM staining for ECL.
Purpose: To examine tissue loss rates, processing time, and primary graft failure (PGF) of "prestripped-only" Descemet membrane endothelial keratoplasty (DMEK) grafts at a single eye bank and how these parameters changed after the introduction of steps to preload tissue among experienced processors.
Methods: Tissue loss and processing time during DMEK graft preparation as well as PGF were analyzed retrospectively at a single eye bank between 2012 and 2018. Outcomes were assessed in consecutive grafts before and after the introduction of preloading to the eye bank's standard operating procedure.
Purpose: Previous work has suggested that Descemet membrane (DM) folds arise in response to corneal swelling. However, their origin has not been closely explored. In this study, we used optical coherence tomography to evaluate whether DM folds arise secondary to folds in the middle stroma.
View Article and Find Full Text PDFPurpose: To quantify endothelial cell loss (ECL) caused by orientation stamps on prestripped and preloaded Descemet membrane endothelial keratoplasty (DMEK) grafts, and to examine a method for reducing ECL using a smaller stamp.
Methods: Ten prestripped and 10 preloaded DMEK grafts were prepared with S-stamps. Ten additional preloaded DMEK grafts were prepared with both an S-stamp and a smaller F-stamp in different paracentral areas of the graft.
Article Synopsis
- The study aimed to assess the quality and feasibility of preloaded DMEK grafts prepared by eye bank technicians for shipping to surgeons.
- Using specular microscopy and other methods, the researchers measured endothelial cell loss (ECL) in preloaded grafts and compared it to that from non-preloaded grafts, finding that preloading resulted in higher ECL.
- The results indicated that preloaded DMEK grafts could be effectively evaluated and shipped while complying with medical standards, ensuring surgeons receive accurate information about the tissue quality.
Purpose: To present 6-month clinical outcomes from a series of 165 consecutive Descemet membrane endothelial keratoplasty (DMEK) procedures before and after the introduction of a novel stromal-sided S-stamp preparation technique that has decreased the incidence of iatrogenic primary graft failure by eliminating upside-down grafts.
Design: Retrospective nonrandomized comparative case series.
Participants: We included 165 consecutive eyes that had undergone DMEK surgery for Fuchs' or pseudophakic bullous keratopathy.
Purpose: The aim of this study was to investigate the use of a femtosecond laser (FL) in the eye bank preparation of corneas for Descemet membrane (DM) automated endothelial keratoplasty (fDMAEK) and to compare endothelial cell death in graft preparations between fDMAEK, Descemet stripping endothelial keratoplasty (DSEK), and DM endothelial keratoplasty (DMEK).
Methods: Twenty cadaveric tissues were used to test the fDMAEK method. A 9.
Purpose: To assess the results of a single eye bank preparing a high volume of Descemet membrane endothelial keratoplasty (DMEK) tissues using multiple technicians to provide an overview of the experience and to identify possible risk factors for DMEK preparation failure.
Design: Cross-sectional study.
Methods: setting: Lions VisionGift and Wilmer Eye Institute at Johns Hopkins Hospital.
Purpose. To evaluate Fourier-domain optical coherence tomography (FD-OCT) as an adjunct to traditional slit lamp examination of donor corneas with suspected Anterior Stromal Opacities. Methods.
View Article and Find Full Text PDFPurpose: To evaluate the Descemet stripping automated endothelial keratoplasty (DSAEK) graft deturgescence in preservation medium after microkeratome cut using Fourier domain optical coherence tomography.
Methods: The central and peripheral thickness of DSAEK grafts was measured by Fourier domain optical coherence tomography immediately after microkeratome cuts and 1, 2, 3, and 4 hours afterward. All measurements were taken when the grafts were stored in 4°C preservation medium.