Comparison of human amniotic membrane decellularisation approaches for hESC-derived RPE cells culture.

Objective: Recent clinical studies have shown that the transplantation of functional retinal pigment epithelium (RPE) cells can prevent the onset of RPE degeneration in age-related macular degeneration. This study aimed to investigate the potential of human amniotic membrane (hAM) as a viable scaffold for the growth and proliferation of pluripotent-derived RPE cells.

Methods And Analysis: Three enzymatic hAM de-epithelialisation methods (thermolysin, trypsin-EDTA and dispase II) were assessed by histological analysis and optical coherence tomography (OCT).

Study of the force dynamics at the capsular interface related to ciliary body stimulation in a primate model.

Purpose: To study the mechanical dynamics between the zonuli-capsular complex and an accommodating intraocular lens mechanics simulator using pharmacological stimulation of the ciliary body in a primate eye.

Methods: Following phacoemulsification removal of the lens, force/movement gauges simulating the accommodating intraocular lens mechanics were implanted in 8 primate eyes (4 positioned in-the-bag and 4 on-the-bag). The gauge readings were taken under full effect of pilocarpine 4% or atropine 1% starting 6 weeks after surgery and following 3-month intervals for 30 months.

Visual and accommodative outcomes 1 year after implantation of an accommodating intraocular lens based on a new concept.

Purpose: To evaluate visual outcomes and accommodative gains 1 year after implantation of the NuLens accommodating intraocular lens (IOL).

Setting: Department of Refractive Surgery, Vissum Corp., Alicante, Spain.

The NuLens accommodating intraocular lens.

The NuLens intraocular lens (IOL) uses a new optomechanical concept to generate a wide range of dioptric power from minimal movements of the ciliary apparatus during accommodative effort. Basic study of the concept of optomechanics in the primate eye led to successful construction and implantation of functioning IOL prototypes.

Feasibility and development of a high-power real accommodating intraocular lens.

Purpose: To develop an accommodating intraocular lens (IOL) that changes power with ciliary body action and generates a minimum of 8.0 diopters (D) by manipulation of a flexible material between a sulcus-fixated rigid plate and a ciliary muscle-operated capsular diaphragm.

Setting: Vissum-Instituto Oftalmológico de Alicante and Miguel Hernández University, Alicante, Spain.

Retinal microvascular patency in the diabetic rat.

Purpose: To study whether the patency to erythrocytes in retinal microvessels of diabetic rats is reduced or blocked before the vessels lose their patency to plasma flow.

Methods: We used recognized techniques to induce diabetic and galactose related microvascular retinal lesions in rats: (1) alloxan induction (2) streptozotocin induction (3) galactose-containing diet. The rats were followed up to 17 months.

Nucleus cryoinversion for extraction of highly complicated cataracts.

We describe a technique for cataract extraction in vitrectomized eyes that uses secured removal of the nucleus with no manipulation of the capsular bag. After a large scleral tunnel is prepared and a capsulorhexis created, a 20-gauge retinal cryoprobe enveloped in a plastic or a silicone sleeve is introduced into the anterior chamber. The cryoprobe engages the nucleus by a "freeze-grip" and rotates it 180 degrees so that the nucleus is elevated into the anterior chamber with no force applied to the capsule and the cryoprobe supporting it from below.

Endoscopic photocoagulation of the ciliary body after repeated failure of trans-scleral diode-laser cyclophotocoagulation.

Purpose: To report the successful control of intraocular pressure with endoscopic cyclophotocoagulation after repeated failure of trans-scleral diode-laser cyclophotocoagulation.

Design: Interventional case report.

Methods: A 3 1/2-year-old child had intraocular pressure around 30 mm Hg in one eye despite full medical treatment and three procedures of trans-scleral diode laser photocoagulation.