Immobilization of Paenibacillus polymyxa with biopolymers to enhance the production of 2,3-butanediol.

Background: Paenibacillus polymyxa, is a Gram-positive, plant growth promoting bacterium, known for producing 98% optically pure 2,3-butanediol, an industrially valuable chemical for solvents, plasticizers and resins. Immobilization of Paenibacillus polymyxa has been proposed to improve the cell stability and efficiency of the fermentation process, reduce contamination and provide easy separation of butanediol in the culture broth as compared to conventional bioprocesses. This research aimed to explore the potential of Paenibacillus polymyxa with immobilization technique to produce 2,3-butanediol.

Gene Silencing via Ingestion of Double-Stranded RNA in Wireworm of Species.

Wireworms are the most destructive soil insect pests affecting horticultural crops. The damage often renders them unsuitable for commercial purposes, resulting in substantial economic losses. RNA interference (RNAi) has been broadly used to inhibit gene functions to control insect populations.

Co-Creating a Vision for the Future of Healthcare Leadership: An Organizational Case Study.

This qualitative case study explores the perspectives of approximately 270 leaders at all levels within Canada's largest community hospital to identify the qualities of effective leaders and their aspirations for the future of healthcare leadership. Responses to a structured visioning exercise with three open-ended prompts were anonymously collected, sorted using ChatGPT and thematically analyzed. Connection, integrity and impact were identified as central to effective leadership.

Unleashing the role of potential adjuvants in leishmaniasis.

Leishmaniasis is amongst one of the most neglected tropical disease, caused by an intracellular protozoan of genus Leishmania. Currently, the most promising strategy to combat leishmaniasis, relies on chemotherapy but the toxicity and increasing resistance of the standard drugs, presses the demand for new alternatives. Immunization is arguably the best strategy for cure because an individual once infected becomes immune to the disease.

Masu salmon species complex relationships and sex chromosomes revealed from analyses of the masu salmon (Oncorhynchus masou masou) genome assembly.

Masu salmon (Oncorhynchus masou) are the only Pacific salmon endemic to Asia. Some researchers prefer to categorize these salmon into four subspecies (masu - O. m.

Case 331: Cardiac Sarcoidosis.

A 43-year-old male patient with no known past medical history presented to the emergency department with new-onset bitemporal headache, dizziness, and bilateral lower extremity weakness for 1 day. The patient denied chest pain, shortness of breath, cough, or recent exposure to sick individuals. He was not on any medications and denied alcohol or illicit drug use.

Assessment of fluoride level in drinking water sources in Sudurpaschim Province, Nepal.

Background: Fluoride, a naturally occurring element found in varying concentrations in water, confers preventive effects in dental caries around a concentration of 1 ppm. The study aimed to assess the level of fluoride in drinking water sources in the Sudurpashchim Province of Nepal.

Methods: A cross-sectional study was conducted from April to July 2023 with water samples from natural, municipal, and packaged sources collected in polypropylene plastic bottles from all 9 districts of the province.

Significance of LIF/LIFR Signaling in the Progression of Obesity-Driven Triple-Negative Breast Cancer.

American women with obesity have an increased incidence of triple-negative breast cancer (TNBC). The impact of obesity conditions on the tumor microenvironment is suspected to accelerate TNBC progression; however, the specific mechanism(s) remains elusive. This study explores the hypothesis that obesity upregulates leukemia inhibitory factor receptor (LIFR) oncogenic signaling in TNBC and assesses the efficacy of LIFR inhibition with EC359 in blocking TNBC progression.

Transforming pediatric healthcare: a decade of innovation in global hospitals (2015-2024).

Background: As pediatric hospitals continue to grow in innovation, it is important to create a standardized metric to measure this growth and create a network to encourage further innovation. We worked to classify all pediatric hospitals on a scale of innovation with the goal of encouraging collaboration that can lead to more overall innovation.

Methods: All pediatric hospitals worldwide were ranked on a scale of innovation from 1 to 3, with 1 being the most innovative, or "established," and 3 being the least innovative, or "nascent.

De novo transcriptome assembly and differential gene expression analysis in different developmental stages of Agriotes sputator (click beetle).

Wireworms, the larva of click beetle (Agriotes species), are one of the most destructive pests of horticultural crops in North America, responsible for considerable economic losses in Canada. Agriotes sputator (A. sputator) species is a predominant wireworm pest attacking potato fields in Eastern Canada.

Human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) for modeling cardiac arrhythmias: strengths, challenges and potential solutions.

Ion channels and cytoskeletal proteins in the cardiac dyad play a critical role in maintaining excitation-contraction (E-C) coupling and provide cardiac homeostasis. Functional changes in these dyad proteins, whether induced by genetic, epigenetic, metabolic, therapeutic, or environmental factors, can disrupt normal cardiac electrophysiology, leading to abnormal E-C coupling and arrhythmias. Animal models and heterologous cell cultures provide platforms to elucidate the pathogenesis of arrhythmias for basic cardiac research; however, these traditional systems do not truly reflect human cardiac electro-pathophysiology.

Phylogenetic analysis linked fatal neurologic disease in leopards (Panthera pardus) to Asia-5 lineage of canine distemper virus in Nepal.

Canine distemper virus (CDV) is responsible for a highly contagious and often fatal neurological disease that affects various carnivores, including domestic dogs. In Nepal, recent reports of CDV exposure and illness in leopards (Panthera pardus) have raised concerns about the transmission of the virus among domestic dogs and wild carnivores. To investigate the genetic lineage and spread of CDV, our study utilized archived post-mortem samples from four leopards that exhibited clinical signs suggestive of canine distemper infection.

Reverse pharmacology based clinical protocols for noninvasive integrative management of low grade cervical precancer lesions: Rationale and outcomes.

Prevention is the most efficient and cost-effective method to combat cervical cancer for which High Risk Human Papilloma Virus (HR-HPV) infection is identified as the major causative factor. HPV vaccination is for primary prevention whereas surgical ablation of precancer is for secondary prevention after HPV infection has occurred. Screening of women for early detection of Squamous Intraepithelial Lesions (SILs) with Papanicolou smear (Pap smear) is a desirable pre-requisite.

Introducing AOD 4: A dataset for air borne object detection.

This paper introduces an airborne object dataset comprising 22,516 images categorizing four classes of airborne objects: airplanes, helicopters, drones, and birds. The dataset was compiled from YouTube-8 M, Anti-UAV, and Ahmed Mohsen's dataset hosted on Roboflow. Videos were sourced from the first two platforms and converted into individual frames, whereas the latter dataset already consisted of images.

Case 331.

A 43-year-old male patient with no known past medical history presented to the emergency department with new-onset bitemporal headache, dizziness, and bilateral lower extremity weakness for 1 day. The patient denied chest pain, shortness of breath, cough, or recent exposure to sick individuals. He was not on any medications and denied alcohol or illicit drug use.

mTORC1 activity oscillates throughout the cell cycle, promoting mitotic entry and differentially influencing autophagy induction.

Mechanistic Target of Rapamycin Complex 1 (mTORC1) is a master metabolic regulator that is active in nearly all proliferating eukaryotic cells; however, it is unclear whether mTORC1 activity changes throughout the cell cycle. We find that mTORC1 activity oscillates from lowest in mitosis/G1 to highest in S/G2. The interphase oscillation is mediated through the TSC complex but is independent of major known regulatory inputs, including Akt and Mek/Erk signaling.

Implicating clinical utility of altered expression of PTCH1 & SMO in oral squamous cell carcinoma.

Introduction: Oral cancer poses a significant burden on public health in India, with higher incidence and mortality rates. Despite advancements in treatment modalities, prognosis remains poor due to factors such as localized recurrence and lymph node metastasis, potentially influenced by cancer stem cells. Among signaling pathways implicated in CSC regulation, the Hedgehog pathway plays a crucial role in oral squamous cell carcinoma (OSCC).

Meiosis-specific functions of kinetochore protein SPC105R required for chromosome segregation in oocytes.

The reductional division of meiosis I requires the separation of chromosome pairs towards opposite poles. We have previously implicated the outer kinetochore protein SPC105R/KNL1 in driving meiosis I chromosome segregation through lateral attachments to microtubules and coorientation of sister centromeres. To identify the domains of SPC105R that are critical for meiotic chromosome segregation, an RNAi-resistant gene expression system was developed.

Comparative evaluation of salivary, serum and urinary 8-OHdG in gutka-associated oral submucous fibrosis.

Background: Gutka chewing is the most common deleterious oral habit prevalent in the geographical distribution of the Indian subcontinent. Gutka leads to the production of numerous free radicals, which causes oxidative stress in regional oral tissues. Oxidative stress brings about the oxidation of guanine bases of DNA that generates 8-OHdG as its main byproduct.

Endothelial ERG programs neutrophil transcriptome for sustained anti-inflammatory vascular niche.

Unlabelled: Neutrophils (PMNs) reside as a marginated pool within the vasculature, ready for deployment during infection. However, how endothelial cells (ECs) control PMN extravasation and activation to strengthen tissue homeostasis remains ill-defined. Here, we found that the vascular ETS-related gene (ERG) is a generalized mechanism regulating PMN activity in preclinical tissue injury models and human patients.