The utility of the hematoxylin and eosin staining in patients with suspected Hirschsprung disease.

Background: While immunohistochemistry (IHC) methods have been widely conducted for the diagnosis of Hirschsprung disease (HSCR) in developed countries, there are very few studies on their use in developing countries where hematoxylin and eosin (HE) staining is a key element of the diagnosis of HSCR. We aimed to determine the accuracy of HE staining in the diagnosis of HSCR using S100 IHC as the reference standard in Indonesia.

Methods: All histopathology performed for the suspicion of HSCR patients from January 2013 to August 2015 in Dr.