Publications by authors named "Joseph Whiteley"
Developing stable biopharmaceutical formulations is of paramount importance and is typically achieved by incorporating surfactants as stabilising agents, such as polysorbate 20 and 80. However, little is known about the effect surfactant grade has on formulation stability. This study evaluates the effect of regular grade and Super-refined™ polysorbates 20 and 80 and their interaction with model proteins, namely β-lactoglobulin (β-Ig), human serum albumin (HSA) and immunoglobulin gamma (IgG), using isothermal titration calorimetry (ITC) and differential scanning calorimetry (DSC).
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- Protein stability is crucial for the effectiveness of biologics, and maintaining their proper 3D structure is essential for therapeutic performance.
- The study introduces a modified UV spectroscopic method to identify protein unfolding, specifically analyzing the effects of polysorbate 20 and polysorbate 80 on model proteins.
- It was found that protein unfolding starts to increase at surfactant concentrations approximately ten times lower than the critical micellar concentration, suggesting this method can help researchers optimize surfactant levels in drug formulations while preventing protein instability.
Article Synopsis
- Post-reperfusion syndrome (PRS) can range from mild to severe during liver transplantation, affecting patient outcomes due to various factors.
- A study found that using a blood flush through a caval vent alongside the standard chilled flush may lead to less hemodynamic instability and fewer metabolic issues.
- Results showed that patients who received the caval flush experienced better mean arterial pressure and heart rate retention, although central venous pressure and lab values were similar between groups, indicating a potential benefit of the technique that warrants further research.
Purpose: Regional anesthesia has been shown to improve outcomes in several recent studies. The transversus abdominis plane (TAP) block provides anesthesia to the abdominal wall by introducing local anesthetic to the ventral rami of the thoracolumbar nerves. This work quantifies the area of anesthesia obtained after performing the novel thoracolumbar interfascial plane block (analogous to the TAP block but intended for the back) which targets the sensory component of the dorsal rami of the thoracolumbar nerves.
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- Robot-assisted laparoscopic radical prostatectomy (RALRP) may elevate intracranial pressure (ICP) due to surgical positioning and pneumoperitoneum.
- A study examined 25 patients and used ultrasonographic analysis of optic nerve sheath diameter (ONSD) to assess ICP before and after surgery.
- Results showed a significant increase in ONSD, indicating an ICP rise above 20 mm Hg during the procedure, with associations found between increased ONSD and mean arterial pressure (MAP).
Background: Acute kidney injury (AKI) is a frequent complication of orthotopic liver transplantation (OLT). Hepatic failure pathophysiology and intraoperative events contribute to AKI after OLT. Colloids are routinely used to maintain intravascular volume during OLT.
View Article and Find Full Text PDFA case of a 49 year old man with a giant basilar artery aneurysm requiring rapid ventricular pacing is presented. Rapid ventricular pacing decreased aneurysm size and increased operative exposure, which aided surgical decision making. It also provided decreased wall tension in the aneurysm.
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