Supported Pt Enabled Proton-Driven NAD(P) Regeneration for Biocatalytic Oxidation.

The utilization of biocatalytic oxidations has evolved from the niche applications of the early 21st century to a widely recognized tool for general chemical synthesis. One of the major drawbacks that hinders commercialization is the dependence on expensive nicotinamide adenine dinucleotide (NAD(P)) cofactors, and so, their regeneration is essential. Here, we report the design of carbon-supported Pt catalysts that can regenerate NAD(P) by proton-driven NAD(P)H oxidation with concurrent hydrogen formation.

A facile analytical method for reliable selectivity examination in cofactor NADH regeneration.

This study demonstrates a novel method to quantify selective (1,4-NADH) and unselective products (1,2- and 1,6-NADH) in NADH regeneration using combined UV-Vis spectroscopy and biological assays. The validity of the proposed method was tested in the Pt/C promoted NAD+ hydrogenation using hydrogen as reducing agent.